Master of Science II (Biotechnology) Examination: Oct Nov 2016
Semester III (New CBCS)
SLR No. Day
Date Time Subject Name Paper No. Seat No.
SLR SK
114
Wednesday
16/11/2016
2:30 P.M
to
5.00 P.M
Advanced Analytical
Techniques
C
IX
Es Instructions: Section-I are compulsory.
Answer any four questions from Section -II
Total Marks: 70
Section- I
Q.1 Rewrite the sentence using correct alternative given below. 07
In circular dichorism, the differential absorption of light
is analyzed.
polarized reflected
inhibited deviated
Radioactive decay is measured in a
Scintillation counter AAS
UV Spectrometer Autoradiography
The first working microscope was designed by
Robert Hook Kepler
Leeuwenhoek Watson
In Western Blotting technique is transferred to the
membrane.
DNA RNA
Protein None
Paper chromatography is a type of chromatography.
Planar column
TLC All of the above
The working rang of a pH meter is in between
8-14 0-14
0-7 1-7
The nuclear fraction is sedimented at
10,000 1000
8000 12,000
Define the following terms: 07
Refractive index
Sedimentation coefficient
Partition coefficient
Isoelectric focusing
pH
Radioactivity
Spectroscopy
Section-II
Q.2 Explain the different types of Electron microscopy with suitable diagrams. 14
Q.3 What are Radio tracer techniques? Give its advantages and restrictions. 14
Q.4 Give the principle, instrumentation, working and applications of Atomic
Absorption Spectroscopy.
14
Q.5 Answer Any two of the following: 14
Explain the technique of Pulse Field Gel Electrophoresis
Write a note on HPLC.
Differentiate between Turbidimetry and Nephelometry.
Q.6 Write shorts note onany TWO of the following: 14
Biosensors
Support material used in the technique of Electrophoresis
Ultracentrifuges