M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Biotechnology
ADVANCED ANALYTICAL TECHNIQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I Compulsory. Answer any four questions from Section II.
Section I
Q.1

Rewrite the sentence after choosing the correct answer from the given alternatives:
07
The nuclear fraction is sedimented at rpm.
10,000
1000
8000
12,000
Radioactive decay is measured in a
Scintillation counter
AAS
UV Spectrometer
Autoradiography
The first working microscope was designed by
Robert Hook
Kepler
Leeuwenhoek
Watson
In Western Blotting technique is transferred to the membrane.
DNA
RNA
Protein
None
Paper chromatography is a type of chromatography.
Planar
Column
TLC
All of the above
The working range of a pH meter is in between
8-14
0-14
0-7
1-7
In circular dichorism, the differential absorption of light is analyzed.
Polarized
Reflected
Inhibited
Deviated

Define the following terms.
07
Refractive index
Sedimentation coefficient
Partition Coefficient
Isoelectric focusing
pH
Radioactivity
Spectroscopy
Page 2 of 2
SLR-UE-103
Section II
Q.2
Explain in detail Electron Microscopy and its types with the help of suitable diagrams.
14
Q.3
Write a note on the advantages and restrictions of Radio tracer techniques.
14
Q.4
Comment on the different types of Rotors with the help of suitable diagrams.
14
Q.5
Answer any two from the following:
14
a)Pulse Field Gel Electrophoresis
b)Write a note on HPLC.
c)Write any five differences between Turbidimetry and Nephalometry.
Q.6
Write short notes on any two of following:
14
a)Applications of MALDI Tof in Biological sciences.
b)Explain the technique of detection of ionization in Geiger Muller counter.
c)Use of centrifuges for molecular weight determination.