M.SC. II (Semester III) (CBCS) Examination, 2017
BIOTECHNOLOGY
Advanced Analytical Techniques
Day Date: Tuesday, 19-04-2017 Max. Marks: 70
Time: 02.30 AM to 05.00 PM
NOTE
Section-I Compulsory
Answer any four questions from Section- II
Section-I
Q.1 Rewrite the sentence using correct alternative given below. 07
In circular dichorism, the differential absorption of light
analyzed.
Polarized Reflected Inhibited Deviated
Radioactive decay is measured in a
Secintillation counter AAS
UV Spectrometer Autoradiography
The first working microscope was designed by
Robert Hook Kepler Leeuwenhoek Watson
In Western Blotting technique is transferred to the
membrane.
DNA RNA Protein None
Paper chromatography is a type of chromatography.
Planar Column TLC All of the above
The working range of a pH meter is in
8-14 0-14 0-7 1-7
The nuclear fraction is sedimented at
10,000 1000 8000 12,000
Define the following terms: 07
Numerical Aperture
ii) RCF
iii) Mobile phase in Chromatography
iv) Capillary electrophoresis
Electromagnetic Radiation
vi) Scintillation
vii) Electrode
Page 1 of 2
SLR-RD 127
Section- II
Q.2 Comment on the different types of Rotors with the help of suitable diagrams. 14
Q.3 Write a note on the methods of measurement of Radioactivity? Give their
advantages and restrictions.
14
Q.4 Give the principle, instrumentation, working and applications of UV-Vis
Spectroscopy.
14
Q.5 Answer any TWO of the following: 14
Explain the technique of SDS-PAGE.
ii) Write a note on GC-MS.
Iii) Explain the technique of NMR.
Q.6 Write short notes on ant TWO of the following: 14
Applications of radio isotopes in Biological sciences.
ii) Support material used in the technique of Chromatography.
iii) Use of centrifuges for molecular weight determination.