M.Sc. (Semester III) (CBCS) Examination Mar/Apr-2018
Biotechnology
ADVANCED ANALYTICAL TECHINQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I is compulsory. Answer any four questions from Section II.
Section I
Q.1

Rewrite the sentence after choosing the correct answer from the given alternatives:
07
The name of the protein staining dye is
Safranine
Diphenyle amine
Basic fuchsin
Coomasie brilliant blue
The used in centrifugation is a self forming gradient.
Ficoll
Sucrose
Maltose
All of the above
In electron microscopy the source of illumination is
Light
Tungsten filament
Nichrome wire
Black Rod
The is used as a ligand in affinity chromatography of antibodies.
Substrate
Antigen
PEG
All of the above
The membrane used in blotting of DNA is membrane.
Dialysis
Nitrocellulose
Polyethylene
All of the above
In circular dichorism, the differential absorption of light is analyzed.
Polarized
Reflected
Inhibited
Deviated
Radioactively labeled nucleotides can be visualized in situ by
Flow cytometer
AAS
UV Spectrometer
Autoradiography

Define the following terms.
07
Electrode
RCF
Stationary phase in Chromatography
Capillary electrophoresis
Electromagnetic Radiation
Scintillation
Refractive index
Page 2 of 2
SLR-UE-97
Section II
Q.2
Write a note on the methods of measurement of Radioactivity? Give their advantages and restrictions.
14
Q.3
Give the principle, instrumentation, working and applications of Atomic Absorption Spectroscopy.
14
Q.4
Explain the different types of Electron microscopy with suitable diagrams.
14
Q.5
Answer any two from the following:
14
a)Write a note on GC-MS.
b)Explain the technique of SDS-PAGE.
c)Explain the technique of NMR.
Q.6
Write short notes on any two of following:
14
a)Ultracentrifuges
b)Support material used in the technique of Chromatography.
c)Applications of radio isotopes In Biological sciences.