M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Biotechnology
ADVANCED ANALYTICAL TECHNIQUES
Time: 2½ Hours Max. Marks: 70
Instructions: Section I compulsory.
Answer any four questions from section II.
SECTION I
Q.1 Rewrite the sentence using correct alternative given below: 07
In circular dichorism, the differential absorption of light is
analyzed.
Polarized Reflected
Inhibited Deviated
Radioactive decay is measured in a
Scintillation counter AAS
UV spectrometer Autoradiography
The first working microscope was designed by
Robert Hook Kepler
Leeuwenhoek Watson
In Western Blotting technique is transferred to the membrane.
DNA RNA
Protein None
Paper chromatography is a type of chromatography.
Planar Column
TLC All of the above
The working range of a pH meter is in between
8-14 0-14
0-7 1-7
The nuclear fraction is sedimented at rpm.
10,000 1000
8000 12,000
Define the terms:- 07
Refractive index
Sedimentation coefficient
Partition coefficient
Isoelectric focusing
pH
Radioactivity
Spectroscopy
SECTION II
Q.2 Explain the scanning Electron microscopy with suitable diagrams. 14
Q.3 What is Radioactivity? Give the working of Scintillation counting. 14
Q.4 Give the principle, instrumentation, working and applications of Infra Red
Spectroscopy.
14
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SLR-VE-84
Q.5 Answer any two of the following: 14
Explain the technique of Capillary Electrophoresis.
Write a note GC-MS.
Write a note on UV V is Spectrophotometry.
Q.6 Write short notes on any two of the following:- 14
X-ray Crystallography
Applications of radio isotopes.
Ultracentrifuges