M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Biotechnology
ADVANCED ANALYTICAL TECHNIQUES
Day Date: Thursday, 16-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Section I is compulsory.
Answer any Four questions from section II.
Section I
Q.1 Rewrite the sentence after choosing the correct from the given 07
Radioactively labeled nucleotides can be visualized in situ by
Flow cytometer AAS
UV Spectrometer Autoradiography
2 The used in centrifugation is a self forming gradient.
Ficoll Sucrose
Maltose All of the above
3 In electron microscopy the source of illumination is
Light Tungsten filament
Nichrome wire Black Rod
4 The is used as a ligand in affinity chromatography of
antibodies.
Substrate Antigen
PEG All of the above
5 The membrane used in blotting of DNA is membrane.
Dialysis Nitrocellulose
Polyethylene All of the above
6 In circular dichorism, the differential absorption of light is
analyzed.
Polarized Reflected
Inhibited Deviated
7 The name of the protein staining dye is
Safranine Diphenyle amine
Basic function Coomasie brilliant blue.
Define the following terms:- 07
Refractive index
RCF
Stationary phase in Chromatography
Capillary electrophoresis
Electromagnetic radiation
Scintillation
Electrode
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SLR-ME-109
Section II
Q.2 Explain the different types of Electron microscopy with suitable diagrams. 14
Q.3 Write a note on the methods of measurement of Radioactivity? Give their
advantages and restrictions.
14
Q4 Give the principle, instrumentation, working and applications of Atomic
Absorption Spectroscopy.
14
Q5 Answer any two of the following. 14
Explain the technique of SDS-PAGE.
Write a note on GC-MS.
Explain the technique of NMR.
Q.6 Write short notes on any two of the following:- 14
Application of radio isotopes in Biological sciences.
Support material used in the technique of Chromatography.
Ultracentrifuges