1. Fatty acid methyl esters of varying carbon lengths can be separated by

Gas chromatography

Affinity column chromatography

Sucrose density gradient centrifugation

Ion exchange chromatography



Positively charged basic amino acids are

Lysine and arginine

Lysine and asparagine

Glutamine and arginine

Lysine and glutamine


If the Standard Gibb's free energy, for a reaction is positive then

the products will be favored

the reactants will be favored

the concentration of the reactants and products will be equal

all of the reactant will be converted to product


Succinate is converted to fumarate by succinate dehydrogenase. In the presence of reversible competitive inhibitor, malonate in place of succinate, the enzyme's

KIn decreases and Vmax remains the same

both KIn and Vmax decreases

KIn increases and Vmax remains the same

both KIn and Vmax increases


Which one of the following inhibits transcription?

Rifampicin

Chloremphenicol

Novobiocin

Kanamycin


A person's broca's area on his left hemisphere was affected when he experienced a stroke. What ability could be affected?

understanding what others say

being able to speak

being able to touch his nose

reading


Sex lethal in Drosophila is transcribed when

the X autosome balance is less than 0.5

the X autosome balance is 1 or greater

the X autosome balance equals 0.5

none of the above

A synchronous culture is one in which the majority of cells proceed through

Lag phase

Log phase

Exponential phase

Each cell cycle phase G G2 and


Which of the following statement is false:

Large insoluble or aggregate macromolecules are more immunogenic

D-amino acid polymers are more immunogenic

L amino acid polymers can be easily degraded within APCs

B cell only recognizes antigen presented by class I or Class II molecules


10) One of the following is not molecular descriptor

Molecular weight

LogP

Molecular absorption

Refractive index


11) Cells that release histamine and other vasoactive substances in response to allergens are:

Neutrophils

macrophages

NK cells

mast cells


12) One of the following nucleotide substitution in the coding part of DNA is an example of synonymous substitution

TCC to TCA (Serine to Serine)

GAC to CAC (Aspartate to Arginine)

TGG to TGA (Tryptophan to stop-codon)

GAA to GCA (Glutamate to Alanine)


13) When the power of ocular lens is 10 x and objective lens is 20 the magnification is

30 times

20 times

200 times

2000 times


14) The following are true about antigen presenting cells EXCEPT:

Langerhan cells are the antigen presenting cells of the epidermis

Expresses co stimulatory molecules

CD8+ cells only recognize antigen presenting cells bearing MHC I molecules

Follicular dendritic cells are professional antigen presenting cells.



15) Which of the following occur in the presence of glucose?

lac Z gene expression is increased

cAMP increases

Binding of CAP-cAMP complex to the promoter area decreases

none of the above


16) SDS is used in polyacrylamide gel electrophoresis of a mixture of proteins for their efficient separation on the gel. SDS, in this experiment, is used to

stabilize the protein

solubilize the protein

decrease the surface tension of buffer

have uniform charge density on the proteins


17) The cells recognizes antigenic peptides:

only in the free form

only when loaded onto MHC molecule

only when bound to hapten

only when bound by antibodies


18) Which of the following mutations is most likely to be disruptive to protein synthesis or function?

UAUtoUAC

UAUtoUUU

UAUtoUAA

UAUtoCAU


19) Which of the following separation method is best suited for a protein sample with large differences in molecular mass?

Dialysis

Salting out process

Isopycnic centrifugation

Rate zonal centrifugation


20) Cell that has higher mitochondrial activity

Lymphocytes

RBC

Neuron

Fibroblast


21) CD antigens

allow leukocytes to recognize antigen

are each expressed on only one cell type

are expressed on immune cells to mark them for separation

function as receptors for cytokine and CAMs

22) Spectroscopy measures the change in behaviour of a molecule when it is exposed to

Centrifugal force

Acidic conditions

An electrical charge

Electromagnetic radiation


23) Following is a ligand-based drug design approach:

Docking

QSAR

Dynamics

Monte Carlo


24) Among the following, which vector-borne disease is not transmitted by the mosquitoes?

Chikungunya

Leishmaniasis

Lymphatic filariasis

Japanese encephalitis


25) Which one of the following amino acid is NOT post-translationally modified by
phosphorylation

Glycine

Serine

Threonine

Tyrosine




26) The mechanism by which a pH gradient across a membrane is used to drive an energy requiring process such as the rotation of bacterial flagella

Proton-motive force

Chemiosmotic coupling

Oxidative phosphorylation

Electrochemical proton gradient


27) The pH of inter-membrane space, stroma and thylakoid lumen are

7.5 and 7.0

8,5 and 7.5

7,7.5and5

7,5.0 and 7.5


28) Specific solutes move through the membrane much more slowly via transports than by channels. This is because

Transporters must bind the solute and undergo a series of conformational changes
to transfer the solute across the membrane

Transport through channels is much faster because they are ion specific pores that neither bind the ion nor undergo any conformational changes in order to move it across the membrane.

Transporters undergo a series of conformational changes and must bind the solute to transfer the solute across the membrane.

BothAandB


29) Which of the following participate in cell signaling despite present in very small quantities in the plasma membrane of mammalian cells

Phosphatidylserine

Phosphatidylinositol

Sphingomyelin

Phosphatidylethanolamine


30) A technique which monitors the closeness of two fluorescently labelled molecules and their interactions in cells is

NMR

FRET

Western blotting

Two-hybrid system


31) Which one of the following properties of an enzyme is responsible for its saturation behaviour that is, a maximum rate insensitive to increasing substrate concentration?

The energy does not change the overall equilibrium constant for a reaction.

The enzyme has a fixed number of active sites where substrate binds.

The product of the enzyme reaction usually inhibits the enzyme.

The enzyme lowers the activation energy of a chemical reaction.


32) The half-life of one of the following radioactive isotopes is 5730 years. Identify it

35S

³H

14C

³²p


33) The specific activity of an enzyme would be reported in which of the following units
of measure?

Millimoles per liter

Units per milligram

Micromoles per minute

Milligrams per micromole


34) Which of the following monosaccharides is not a carboxylic acid?

6-phospho-gluconate

Gluconate

Glucose

Glucuronate


35) Arrange in sequence the following events that are performed during the isolation of genetic material in recombinant DNA technology

1.Removal of DNA by spooling

2.Precipitation of DNA

3.Dissolution of biological membranes

4. Enzymatic digestion











36) Cerebral aqueduct is associated with

Broca's area

Wernicke's area

Angular gyrus

Ventricles


37) Deficiency of thymus results in increased infections in humans such a condition is called:

Thymectomy

Graves disease

Di Georges syndrome

All the above


38) A redox reaction in the citric acid cycle whith do not use NAD+ to accept electrons

Oxidation of Fumarate

Oxidation of Succinate

Oxidation of Citrate

Oxidation of Oxaloacetate


39) The type of silencing involved in miRNA is

Transcriptional

Translational

Post transcriptional

Post translational


40) Which of the following is an epimeric pair?

D-glucose and D-glucosamine

D-glucose and D-mannose

D-lactose and D-sucrose

L-manno"se and L-fructose


41) What type of enzyme is used in recombinant DNA technology to split a specific sugar phosphate bond in each strand of a DNA double helix?

Esterase

Restriction enzyme

Lipase

Ligase


42) Which of the following detergent is commonly used to release integral proteins from its membranes?

Urea

Dimethyl sulphoxide

Triton X 100

Cyanogen bromide


43) In which of the following separation method where proteins are separated on the basis of their net charge

Ion exchange chromatography

Affinity chromatography

Dialysis

Gel filtration chromatography


44) Which of the following can precipitate antigens:

Fab

Fc

F(ab)a

All


45) One of the following molecule binds to major grove of DNA

Ethidium bromide

Distamycin

Cisplatin

Methyl green


46) You have been asked to PCR amplify a specific sequence from cDNA that was synthesized from mRNA isolated from brain tissue. After you run your potential PCR product on an agarose gel, you observe no bands when you visualize the gel using ultraviolet light. Why might this be the case?

The gene you are interested in is not expressed in brain tissue.

You used poly dT instead of poly dA to prime the first strand cDNA synthesis.

You used RNAse H instead of DNAse H to leave behind small segments of RNA
to.prime the second strand cDNA synthesis reaction.

You used reverse transcriptase instead of DNA polymerase to synthesize the first strand of cDNA.


47) The specificity of a ligand binding site on a protein is based on

the absence of competing ligands

the amino acid residues lining the binding site

the presence of hydrating water molecules

the opposite chirality of the binding ligand



48) Staphylococci causes

Food intoxication

Food Infection

Food preservation

Food quality improvement



49) a-amylase is produced by

Saccharomyces cerevisiae

Clostridium acetobutylicum

Bacillus subtilis

Saccharomyces cerevisiae



50) An enzyme was purified to 1500-fold by applying different chromatographic steps to the 70% ammonium sulphate precipitated crude protein. If the enzyme is pure at this stage what fraction of the protein in the cell does it constitute?

70%

0.07%



30%


51) Catenanated dsDNA molecules can be resolved leased by

Topoisomerase I

Helicase

Resolvas

Topoisomerase II



52) Non-covalent pairing intermediate formed during homologous strand exchange is called

Joint molecule

dsDNA

ssDNA

Triplex DNA


53) RecA bound helical nucleoprotein is formed through cooperative interaction with

G-protein

single strand binding protein

Beta-protein

SI-protein

54) Following is a classification method

Support vector machine

Simulated annealing

Fragment assembly

Topological shapes


55) During splicing, which of the following snRNPs bind to a nucleotide sequence found in introns called the branch site.

VI

V2

V4 and U6

V5


56) Following species escape from immune recognition

Protein

Peptide

1000 nm particle

400 nm particle


57) If one requires to maintain pH at 4.2, which is best buffer

Borate

Acetate

Phosphate

Borax


58) Which part of the brain controls eating, drinking, body temperature and provides a link between the brain and the endocrine system?

Parietal lobes

Temporallobes

Amygdala

Hypothalamus


59) Eukaryotic cells with DNA damage often cease progression through the cell cycle until the damage is repaired. This type of control over the cell cycle is referred to as

Proteosome control

Damage control

Anticyclin control

Checkpoint control



60) Which lobe is connected with thinking, planning and emotional control?

Frontal

Occipital

Temporal

Parietal

61) Different possess of a target receptor and ligand are simulated in

Rigid docking

QSAR

CoMFA

Flexible docking



62) The class of immunoglobulin that can get transported across epithelial cells is

IgG

IgE

IgA

IgM



63) Multiple sclerosis could disintegrate a neuron's which ultimately could
affect the speed of a neural impulse.

Dendrites

Axons

Myelin Sheath

Synapse



64) You have been asked to conduct analyses of histones from active and inactive regions of chromatin, where genes are expressed and repressed respectively. While conducting your analyses, which of the following observations would be
UNEXPECTED?

Methylation of lysine residues in some histones is associated with establishment
of heterochromatin, and results in gene silencing.

Serine residues in the tails of some histones have been phosphorylated, and this is
associated with changes in chromatin activity.

Lysine residues in the histone tails have been serinated, resulting in inactive
chromatin, where genes are not expressed.

Acetylation of lysine residues in some histones is associated with active regions
of chromatin, where genes are expressed.



65) Which one of the following structures is NOT found in the central nervous system?

Spinal cord

Thalamus

Pons

Dorsal root ganglion


66) Signal sequences are part of a protein that

signal folding of the protein

signal the protein synthesis on the ribosomes is ended

transport proteins to other sites within the cell

refold proteins in prion-associated diseases.


67) For specific antigen recognition by T cells,

antigen is bound by a T cell membrane antibody

denaturation of antigen does not reduce epitope recognition

MHC molecules are not required

antigen exposure during T cell maturation is required


68) A virus vaccine that can activate cytotoxic T cells must contain

a high dose of virus particles

an adjuvant to stimulate T cell division

live virus

virus peptides


69) Two couples present to the emergency room with severe nausea, vomiting, and diarrhea. One of the patients admits that she had a dinner party and served a salad containing mushrooms she had picked during a hike in the forest earlier that day. Inhibition of which enzyme or process explains the clinical manifestations of alpha­amanitin poisoning seen in these patients?

RNA polymerase II

RNA polymerase I

RNA polyadenylation

RNA polymerase III


70) The unfolding of regular secondary structure causes

little increase in the entropy of protein

large decrease in the entropy of the protein

no change in the entropy of the protein

large increase in the entropy of the protein


71) Why is it that inhaling nitric oxide reduces blood pressure only in the lung tissue and not elsewhere in the body?

Because other body tissues use a different signaling molecule

Because nitric oxide cannot cross cell membranes and enter the blood

Because nitric oxide breaks down quickly and thus cannot travel far

None of the above


72) Antibiotic that acts by inhibiting bacterial cell wall synthesis

Erythromycin

Puromycin

Amphotericin

Penicillin



73) One of the following is not a prion disease

Scrapie

Bovine spongiform encephalopathy

Creutzfeldt -Jacob Disease

Parkinson's disease


74) In the context of prokaryotic gene expression, which of the following is the most appropriate definition of an operator?

A cJuster of genes that are regulated by a single promoter.

A DNA-binding protein that regulates gene expression.

A non-coding, regulatory DNA sequence that is bound by RNA polymerase.

A non-coding, regulatory DNA sequence that is bound by a repressor protein.


75) The technique used to detect the presence of DNA or RNA in a non-fractionated
DNA sample is

in situ hybridization

colony hybridization

dot blot technique

western blotting technique