1. What is the final pH of a solution made by mixing 100 ml of 0.05 M acetic acid and 100 ml of 0.1 M sodium acetate? Assume the pKa for acetic acid is 4.76.

5.06

5.60

5.43

5.50

2. You have a liquid culture of yeast Saccharomyces cerevisiae. You have diluted it by lOs folds and have plated 0.1 ml of the diluted culture on a solid agar plate to obtain 63 colonies. What was the OD600 of the initial culture? (Given that 10D6oo 3 X 10^7 cells/ml

0.21

0.33

2.1

3.3

3. Three persons Band Cwore shirts of black, blue and orange colors (not necessarily in that order) and pants of green, yellow and orange colors (not necessarily in that order). No persons were shirt and pants of the same color. Further it is given that;

A did not wear shirt of black color

B did not wear shirt of blue color

C did not wear shirt of orange color

A did not wear pant of green color

B wore pants of orange color

What were the colors of the pants and shirt of C respectively?

Orange and Black

Green and Blue

Yellow and Blue

Yellow and Black

4. Which of the following statistical test could be used to calculate and the compare the difference between the mean values of two samples?

t-test

x2-test

F-test

ANOVA

5. In a certain code language means books are old; means man is old and means buy good books. What stands for in that code?

2

4

5

6

6. P+Q means is the brother of P-Q means is the mother of PxQ means is the sister of Which of the following means P is the maternal uncle of







P+RXQ

7. Radha is the niece of Alok. Alok's mother is Priya. Seema is Priya's mother. Seema's husband is Hari. Rita is the mother-in-law of Hari. How is Alok related to Hari.

Son

Daughter's son

Grand nephew;

Great grandson's son

8. If GO=32 and SHE=49, then SOME will be equal to

56

58

62

64

9. Raju travels 10 km towards north, turns left and travels 4 km, and then turns right and covers 5 km more, and then turns right, and proceeds another 4 km. What is the displacement of Raju from the starting point?

5km

10 km

15 km

20km

10.

<img src='./qimages/16031-10.jpg'>

In the above diagram,

Rectangle represents females;

ii) Triangle represents uneducated persons;

iii) Circle represents urban;

iv) Square represents Government employees. In different regions in the diagram some numbers are indicated which corresponds to the persons. How many persons are urban females but not uneducated and Government employees?

14

4

7

6

11. 50% of a number is equal to the three-fourth of another number y. What is the ratio of x and

2:3

3:2

3:4

4:3

12. The total sum of ages of 5 children born at the intervals of 3 years each is 50 years. What is the age of the youngest child?

10 years

8 years

6 years

4 years

13. There are 200 questions in a 3 hr examination. Among these questions 50 are mathematical problems. It is suggested that twice as much time be spent on each math problem as for each other question. How many minutes should be spent on mathematical problems?

36

72

60

100

14.





a^mxn



15. A coin was tossed 1000 times of which the head appeared 455 times, find the probability of the tail.

0.455

0.545

4.55

5.45

16. Ramesh solved 2/7 part of an exercise while Seema solved 4/5 of it. Which of the following statements are true?

Ramesh took lesser time

Seema took lesser time

Both took the same time

None of the statements are true

17. Complete the sequence:

8

9

64

16

18.

10,10

11,13

13,14

13,16

19. For the given right angle triangle, find the value of a.

<img src='./qimages/16031-19.jpg'>

n/6

n/4

n/3

n/2

20. Guess the element in the sequence: OKY, FJW, HIU, JHS,

KFR

KGR

LGQ

LFQ

21. A PCR reaction that continues for 30 cycles will produce how many PCR products from a single template DNA molecule?

60

128,000

Approximately 1 million

Approximately 1 billion

22. RNA interference (RNAi) of a gene regulatory protein was conducted. Following RNAi, the transcriptome of the treated cells was compared to the transcriptome of the untreated cells, using microarray analysis. Surprisingly} no difference in transcriptome activity was observed between treated and untreated cells. Why might this be the case?

The protein is not a gene regulatory protein.

The protein requires co-regulatory proteins that are not present in the cell type tested.

The protein was unable to enter the nucleus on account of the absence of a signal that allows transit to the nucleus.

B and C could account for the observation.

23. When conducting an electrophoretic mobility shift assay using nuclear extracts from muscle cells, you find that you have three shifted bands. Why might this be the case?

Three different DNA-binding proteins have bound to the DNA probe.

One DNA-binding protein binds to the DNA probe} but it can bind as a monomer} a dimer, or a trimeric complex.

One DNA-binding protein binds to the DNA probe, but it exists as three different post-translationally modified forms in the nuclear extract.

and could explain the observation.

24. In the laboratory} you combined wheat germ DNA sample with water and loading dye. What is the function of loading dye in electrophoresis?

It is used as a way of estimating how far your DNA sample has migrated in the gel

It is used to visualize your DNA sample

It increases the density of your DNA sample causing it to sink to the bottom of the well in the gel

a and c

25. You prepared a Master Mix solution for PCR. Which of the ingredients listed below was NOT included in the Master Mix?

Taq polymerase

EDTA

dNTPs

MgCIz

26. Which of the following is TRUE about the restriction enzyme BamH1 The arrow indicates where the enzyme cuts the DNA.

When it cuts DNA, it creates a overhang

When it cuts DNA, it creates a overhang

It is classed as an exonuclease

It can cut both single-and double-stranded DNA

27. Isoelectric focusing of proteins is associated with the technique

Chromatography

Electrophoresis

Crystallography

Spectroscopy

28. Hybridization of single stranded RNA or DNA is facilitated by

high temperature

ii) low temperature

iii) high salt

iv) low salt

the presence of an unrelated DNA

Only ii) and iii) are true

Only and iii) are true

Only ii) and iV) are true

Only ii) and are true

29. In two dimensional polyacrylamide gel electrophoresis second electrophoresis is done at degree angle relative to the first one.

30°

60°

90°

180°

30. Which of the following method is used for the improvement of microbes in industry?

Protoplast fusion

Recombinant DNA technique

Mutation

All of the above

31. In size exclusion chromatography the molecules bigger than a particular size don't get into the matrix

This results in the small molecules entering in matrix and thus they come out first in the process

This results in the small molecules entering in matrix and thus they are retained in the matrix permanently

This results in the big molecules to be excluded from entering into the matrix and they come out last in the process

This results in the big molecules to be excluded from entering into the matrix and they come out first in the process

32. Which of the following act is not considered as an act of Plagiarism?

Representing someone else's work as your own without citing

Changing words but copying the sentence structure of a source without citing

Reusing the identical or nearly identical portions of one's own work without acknowledging that one

Taking passages from other's work using quotations and citing

33. Flow Cytometry is used for the measurement of

DNA content in a cell

Size of cells

Type of cells

All of the above

34. Which of the following is correct in case of microarray technology?

Hybridization of RNA: RNA

Hybridization of labeled and unlabeled cDNAs

Hybridization of RNA: protein

All of the above

35. Number of chromosomes in primary cell culture will

Remain the same

Be doubled

Be tripled

Be increased by several times

36. Repeated centrifugation steps with progressively high speed refers to

High speed centrifugation

Density gradient centrifugation

Differential centrifugation

Equilibrium centrifugation

37. A viruses' plaque forming units indicates

Potential to transmit

Potential to initiate a productive infection

Potential to elicit immunity

Potential to show symptoms

38. Infectious dose 50 (ID50 means the dose required to

Transmit to 50% of the animals

Kill the 50% of the animals

Show the symptoms in 50% of the animals

Infect 50% of the inoculated animals

39. If, circular and open circular DNAs were analyzed in a single agarose gel electrophoresis

Both DNAs migrates at same speed

Circular DNA migrates faster

Open circular migrates faster

No relevance

40. The amino acid that used in sDs-PAGE technique is

Glycine

Proline

Arginine

Serine

41. Which of the following residue/modification combinations is false?

Cysteine and disulphide bridges

Proline and phosphorylation

Asparagine and glycosylation

N-terminus and lipidation

42. Which of the following is the host of a virus?

Fish

Bacteria

Prawns

All

43. In the normal human being the concentration(s) of various antibodies in the serum is in the order:

IgM>lgA>lgG>lgE

IgG>lgA>lgM>lgE

IgE>lgG>lgM>lgA

IgA>lgM>lgE>lgG

44. When comparing ultraviolet light and X-ray,

X-rays have longer wavelengths and are thus less mutagenic

X-rays have longer wavelengths and are thus more mutagenic

X-rays have a shorter wavelength than does UV light

X-rays and UV lights are of the same wavelength and are equally mutagenic

45. An example of innate immunity is

T-Iymphocytes

B-Iymphocytes

Neutrophils

Thyroid cells

46. The precursor(s) for the synthesis of isopentylpyrophosphate in plastids

RuBP and Acetyl CoA

Acetyl CoA

Mevalonyl CoA

Pyruvate and D-glyceraldehyde-3-phosphate

47. Cowpox virus was used as vaccine for smallpox which works on the basis of

Cross reactivity

Same family of viruses

Heterogeneity

None

48. The light activation of ribulose phosphate kinase is facilitated by

Thioredoxin

Flavodoxin

Malate Dehydrogenase

Pyruvate Dehydrogenase

49. Kinase activity is inhibited by which of the following co-factor?

(a)ATP

GTP

ATPY-32

ATPYS

50. Following is not a retroviral replication intermediate

ssRNA

RNA: DNA hybrid

DNA

Duplex RNA

51. Peroxide mediated DNA damage can be detected by the presence of

Double stranded breaks

Single stranded breaks

Oxidized bases

All the above

52. Four different types of clear mutations are found in lambda (the clear phenotype indicates that no lambda Iysogens form): three of these (Iambda_el, lambda_ell, and lambda_clll) are defective in the cl, cll, and clll genes, respectively; the fourth (Iambda_cy) is defective in PRE so that it no longer binds the cll product. Indicate which of these would allow a mutant phage to bypass A immunity and grow on a lambda lysogen?

lambda_cl

lambda_cll

lambda_cy

None of the above

53. Which of the following is not a chaperone?

Hsp70

Bip

HSFl

Hsp40

54. Xeroderma pigmentosum in human is associated with a mutation in

Photoreactivation

Nucleotide Excision Repair

Base Excision Repair

Mismatch Repair

55. Advantage of NMR spectroscopy over X-ray crystallography is

It can be done on macromolecules in solution

It can give dynamics of the atoms in the molecule

You don't need to crystallize the protein

All of the above

56. The intensity of color is used to quantify the differential expression of genes in

Microarray

RNA Seq

SAGE

None of the above

57. Phycobiliproteins are present in

Rhodobacter

Pseudomonas

Xanthomonas

Nostoc

58. The two principal backbone torsion angles for a polypeptide chain are and

jf and X

jf and

and jf

and

59. The whole three dimensional structure of myoglobin is an example for

Protein quaternary structure

Protein primary structure

Protein secondary structure

Protein tertiary structure

60. Antigen-antibody agglutinations occurring during blood transfusions are due to

Cross reactivity

Heterogeneity

Homogeneity

All

61. In mitochondria, the enzyme Succinate Dehydrogenase is associated with

Complex I

Plastoquinone

Complex II

Complex III

62. Which of the following are cytosolic TLRs?

TLR9

TLR3

TLR8

All of the above

63. Microorganism that is being used in Acetone production is

Saccharomyces cerevisiae

Lactobacillus bulgaricus

Clostridium acetobutylicum

Bacillus subtills

64. Which of the following biomolecules can't be synthesized by our body and hence it is required to be supplied or supplemented through our diet?

Omega-6 fatty acids (PUFAs)

Omega-3 fatty acids (PUFAs)

Cholesterol

Triacyl glycerol

65. Which I)f the following set of amino acids consists of only polar side chains?

Ala, Gly, Pro, Phe

Ser, Gly, Pro, Leu

Gly, Lys, Asn, Arg

Ser, Glu, Asp, Thr

66. Protein core (interior/ buried portion) is predominantly comprises of ...... amino acid residues

Polar

Apolar/hydrophobic

Neutral

Charged

67. Which of the following statements regarding collagens is true?

A protein containing a short stretch of Gly-X-Y repeats is technically considered as collagen

Of all the possible combinations only heterotrimers are found in the human extracellular matrix

The propeptides of collagen are cleaved off in the exiting vesicle in order to start creating collagen fibre

The X and Y residues (of the Gly-X-Y repeats) are typically acidic

68. Role of a 'sparger' in a bioreactor is

To supply water

To supply Media

To control pH

To supply air

69. 'Facultative anaerobe' will grow

At the surface of the culture medium

At the bottom of the culture medium

At the middle of the culture medium

Throughout the culture medium

70. Which of the following statements is false?

B cell receptor binds to soluble antigens while T cell receptor binds to antigen presented by MHC

Glycosylation site is present in the Fc region of antibody

D-amino acid polymers are more immunogenic

L-amino acid polymers can be easily degraded within APCs

71. The output of the phylogenetic analysis will be in the form of

Histogram

3D models

Graph models

Dendrograms

72. Which of the following types of RNA contains regions that acts as a switch to turn protein synthesis on and off for the protein it codes for?

microRNA

riboswitches

tRNA

antisense RNA

73. Which one of the following is true for clustered regularly interspaced short palindromic repeats (CRISPR) systems in bacterial cell?

It protects bacteria from virus attack

Codes for cas proteins that can degrade viral mRNA

Can be used to infer a viral infection history

All of the above

74. Which of the following statements concerning hemoglobin are TRUE?

The disease sickle cell anemia results from a mutated hemoglobin gene.

Hemoglobin is composed of 4 identical subunits.

Each subunit of hemoglobin contains a heme group in the site that binds Oz.

The same mutation causing sickle cell anemia is thought to be advantageous (in heterozygotes) against malaria infection.

1,2





4

75. The formation of a phosphodiester bond between two nucleotides:

It is a hydrolysis reaction and requires a hydroxyl group attached to the carbon of the sugar, a phosphate attached to the carbon of the sugar on another nucleotide and an ester bond.

It is a condensation reaction and requires a hydroxyl group attached to the carbon of the sugar, a phosphate attached to the carbon of the sugar on another nucleotide, and an ester bond.

It is a hydrolysis reaction and requires a hydroxyl group attached to the carbon of the sugar, a phosphate attached to the carbon of the sugar on another nucleotide, and an ester bond.

It is a condensation reaction and requires a hydroxyl group attached to the carbon of the sugar, a phosphate attached to the carbon of the sugar on another nucleotide, and an ester bond.

76. In mRNA, refers to

Qne Recognition fragment

Qpen Reading frame

Qne gene Recognized function

Qne time Riding factor

77. Swiss prot is a

Swiss developed genetic engineering technology

Swiss developed plant breeding technology

Swiss developed protein sequence database

Swiss developed floriculture

78. The name 'Ebola virus' is due to

Symptoms

Scientist discovered

Virus family

Place of discovery

79. The genome of Swine flu virus is

Positive sense RNA

Negative sense RNA

Double stranded RNA

Double stranded DNA

80. A scientist has been working to characterize a novel organism from outer space. Recently, the organism's chromatin was examined. Which of the following features of its chromatin would be novel relative to that of most eukaryotes on earth? That is, which one is UNEXPECTED?

147 nucleotide pairs of DNA wrap around a nucleosome core.

The nucleosome core is an octamer comprising pairs of histones H1, H2, H3A, and H3B.

The carboxy terminal tails of some of the histones can be post-translationally modified by the addition of ubiquitin.

There is a linker histone that functions between nucleosome cores.