Master of Science I (Biotechnology) Examination: Oct Nov 2016
Semester II (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SK
103
Saturday
19/11/2016
10.30 AM
To
01.00 PM
Enzyme Technology
C
VI
es Instructions: Section-I is compulsory.
Answer any four questions from Section -II
All questions carry equal marks.
Figures to the right indicate full marks.
Draw neat and labeled diagram.
Total Marks: 70
Section- I
Q.1 Rewrite the following sentences by selecting correct answers from given
alternative.
07
An enzyme which brings about lysis of bacterial cell wall is
Amylase Lysozyme
Trypsin Lipase
Allosteric enzymes regulate the formation of products by
Feedback inhibition Non-competitive inhibition
Competitive inhibition Repression-derepression
Covalent modification of an enzyme usually involves phosphorylation
dephosphorylation of
Serine Proline
Hydroxylysine Hydroxyproline
In competitive inhibitors
Decrease the Km Decrease the Vmax
Increase the Km Increase the Vmax
The CK isoenzyme present in muscle
BB MB
MM None of these
The model of action the anticancer drug methotrexate is through its strong
competitive inhibition on
Dihydrofolate
reductase
Thymidine synthase
thymidine kinase adenylate cyclase
The cooperativity for hemoglobin is
1 1.5
2.8 4
Page 1 of 2
Define the following terms: 07
Specific activity
Ribozyme
Transition state
Enzyme engineering
Steady state enzyme kinetics
Active site
Modulators
Section-II
Q.2 Illustrate the factors affecting catalytic efficiency of enzymes. Add a note on
ribozyme.
14
Q.3 Write an assay on metabolic engineering. 14
Q.4 Explain in detail structure function relationship of enzyme Na ATPase. 14
Q.5 Describe various types of inhibition with their kinetics. 14
Q.6 Answer any TWO of the following: 14
Explain in detail structure function relationship of enzyme Aspartate
Transcarbomylase
Derive Line Michaelis Menten equation. Add a note on signification of Km
and Vmax.
Explain Line weaver Burk plot. Add a note on Characteristics of enzymes.
Explain Scat chard plot. Add a note on enzyme repression.