M.Sc. (Semester II) (CBCS) Examination Mar/Apr-2018
Biotechnology
ENZYME TECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I is compulsory. Answer any Four questions from section II. All question carry equal marks. Draw neat and labeled diagrams. Figures to the right indicate full marks.
Section I
Q.1

Rewrite the sentence after choosing the correct from the given.
07
The degree of inhibition for non-competitive inhibition of an enzyme catalyzed reaction of
Increase with increase substrate concentration
Reaches with increase in substrate concentration
Reaches a maxima with increase in substrate concentration and then decreases
Decreases with increase in substrate concentrate
Enzyme papain is used with success to
Increase meat production
Ripen papaya fruit
Leaven bread
Tenderize meat
Which one of the following reaches used for the purpose of recycling enzymes in bioprocess
Isomerization
Phosphorylation
Immobilization
Polymerization
Which one of the following techniques is not ideal for immobilized cell free enzymes
Physical entrapment by encapsulation
Physical bonding by flocculation
Covalent chemical bonding by cross linking the precipitate
Covalent surface bonding to surface carriers
Most industrial enzymes are obtained from
Plants
Microbes
Insects
Animal tissues
Ki indicate
Reaction velocity
Competition inhibition
Denaturation of enzyme
All the above
Pi indicates
Isoelectric point
Protein indicators
Protein kinetics
None of the above
Page 2 of 2
SLR-UE-90

Define the terms.
07
Isoenymes
ATPase
Bisubstrate reactions
Km
Covalent bonding
Biosensors
Enzyme activators
Section II
Q.2
Explain the multienzyme complex and properties with examples.
14
Q.3
Explain the importance and the derivation of Michaelis-Menten equation.
14
Q.4
What are the functional classifications of enzymes? Explain with suitable Examples.
14
Q.5
Answer any two of the following:-
Clinical aspects of enzymology: LDH isozymes and SGOT.
Effect of temperature, pH and substrate concentration on enzyme.
Biosensors
14
Q.6
Write any two of the following:-
14
Elaborate multienzyme immobilization method.
Define allosteric regulation.
Differentiate between non-competitive and uncompetitive inhibitors.