M.Sc. (Semester II) (CBCS) Examination Nov/Dec-2018
Biotechnology
ENZYME TECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Section I is compulsory.
Answer any four questions from section II.
SECTION I
Q.1 Rewrite the sentences by choosing correct answer from given
alternatives:
07
To synthesize abzymes for hydrolysis of hydroxyester, the is
used as a transition state analogue.
Hydroxyester
Phenol Phosphonate ester
Fatty acid synthesis is an example of
Abzyme Ribozyme
Catmab Multienzyme complex
To study enzyme kinetics by using Hanes-Woolf plot, the values of
are plotted.
Vs V0 Vs
Vs V0 Vs S0
The enzymatic reaction is stopped after incubating for specific period of
time, in kinetic study by the assay.
Continuous Direct
Indirect End point
The amino acids have been found to be critical for catalytic
activity of lysozyme.
Glu35 Asp 52 Glu35 His195
Asp52 Tyr21 His195 Tyr21
The increased levels of in blood, is an indication of liver damage.
Glucose oxidase SGOT
Ribonuclease Trypsin
The ratio of bound to unbound ligand concentration is plotted against
bound ligand concentration in plot.
Eddie Hofstee Hanes
Hills Scatchard
Define the terms:- 07
Turnover number
Multienzyme complex
Metabolic engineering
Biosensor
Cooperativity
Modulator
Covalent modification
Page 2 of 2
SLR-VE-76
SECTION II
Q.2 Describe in detail 'clinical aspects of enzymology.' 14
Q.3 Add a detail account on 'modes of enzyme regulation'. 14
Q.4 Describe various methods of enzyme immobilization. 14
Q.5 Answer any two of the following: 14
Describe graphical procedures in enzymology
Explain catalysis by Na-K ATPases on the basis of structure function
relationship.
Describe 'methods of study fast enzymatic reactions'.
Q.6 Answer any two of the following: 14
Add an account on chemical modification of enzymes.
Describe kinetics of allosteric enzymes based on Hill's and Scatchard plot.
Illustrate the concept of multienzyme complex with examples.