M. Sc. (Biotechnology) (Semester (CBCS) Examination, 2017
ENZYME TECHNOLOGY
wateriI
Day Date: Friday, 21-04-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
N.B. 1)section-I is compulsory.
From section II attempt any four.
All questions carry equal marks.
Figures to the right indicate full marks.
Draw neat and labeled diagram.
Section-I
Q.1 Rewrite the following sentence by choosing the correct
answer.
07
De novo synthesis of an enzyme, promoted by the substrate
on which it acts, is characterized bt the term
Induction Activation
Gratuity Depression
2 Out of total enzymes present in the cell mitochondria above
has
70% 95% No enzymes
3 Enzymes, vitamins and hormones can be classified into a
single category of biological chemicals because all of them.
Aid in regulating metabolism
Are synthesized in organism
Are proteins
Enhance the oxidation metabolism
4 An enzyme which requires a biological change in order to
become active is called
Transferase Zymogen
Hydrogenase Trypsin
5 On top of active site, allosteric enzyme contain
Inhibitors Substrate
Allosteric Site Polypeptide chain
6 Irreversible modification require synthesis of
Enzymes Carbohydrates
Vitamins Proteins
7 Number of substrate molecules converted in to product by
one molecule of enzyme active site per unit time is called
Page 2 of 2
Turnover number Substrate number
Reaction number None of above
Define the following terms: 07
Phosphorylase
Enzyme inhibitors
Importance of V max
IUB system
Ribonuclease
ES complex
CO-enzymes
Section-II
Q.2 Define different methods of immobilization with appropriate examples. 14
Q.3 What is feedback regulation in enzyme kinetics? How does it affect the
enzyme activity.
14
Q4 Elaborate the clinical importance of enzymes for different applications. 14
Q5 Answer any two of the following: 14
Note on Lysozyme and ribonuclease
Micro encapsulation and entrapment method
Significance of V max and K m
Q.6 Answer any two of the following: 14
Describe the importance of end point kinetic assay.
Explain the importance of Hill and Scat chard plot in ligand binding.
Define the kinetics of allosteric enzymes.