M.Sc. (Semester II) (CBCS) Examination Oct/Nov-2017
Biotechnology
ENZYME TECHNOLOGY
Day Date: Monday, 20-11-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Section I is compulsory.
Answer any Four questions from section II.
All question carry equal marks.
Draw neat and labeled diagrams wherever necessary.
Figures to the right indicate full marks.
Section I
Q.1 Rewrite the sentence after choosing the correct from the given 07
In competitive enzyme activity inhibition
The structure of inhibitor generally resembles that of the substrate.
Inhibitor decreases apparent Km
Km remains unaffective
Inhibitor decreases V max without affecting Km
1 international unit is μkayal.
6 60
0.6 600
3 Ternary complex is not formed in
Ordered bi bi reaction Random bi bi reaction
Ping pong bi bi reaction All of these
4 The isoenzymes of LDH
Differ only in a single amino acid
Differ in catalytic activity
Exist in 5 forms depending on M and H monomer contents
Occur as monomers
5 The subunit composition of lactate dehydrogenase of heart is
M4 M2H2
HM3 H4
6 In Na ATPase catalytic activity and ion binding sites are present in
subunit.
β
Both and β None of these
7 Km=
k1+k2/k-1
k-1+k2/k1
Page 2 of 2
SLR-ME-103
Define the terms 07
Turn over number
Km
Ribozyme
SGPT
Metabolic engineering
Cooperativity
Allosteric enzyme
Section II
Q.2 Explain in detail structure function relationship of enzyme Lysozyme. 14
Q.3 Define immobilization of enzyme? Write the methods of immobilization. 14
Q.4 Explain IUB nomenclature system. Discuss in detail enzyme classification
each with two examples.
14
Q.5 What is enzyme inhibition? Explain in detail enzyme inhibition with their
Kinetics?
14
Q.6 Write any two of the following:- 14
Explain in detail structure function relationship of enzyme ribonuclease.
Write a note on bio-sensor.