Master of Science I (Microbiology) Examination: Oct/ Nov 2016
Semester I (Old CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SO
615
Friday
18/11/2016
10:30 AM
to
01:00 PM
Microbiological
Techniques Scientific
Writing
C
II
Instructions: Q.1, Part I is compulsory.
Attempt any four questions from Part II.
Part I and Part II should be written in same answer book.
PART I
Q.1 Rewrite the following sentences by selecting correct answers from given
alternative.
14
An introduction of scientific paper is written in tense.
Present Past
Future all of the above
The most common dye used to make DNA or RNA bands visible for agarose
gel electrophoresis is
Acridine orange Proflavin
Bromothymol blue Eethidium bromide
The most suitable gas to use as a carrier gas in a gas chromatogram is

Methane Oxygen
Helium Oxygen
is a brief summary of the information in a research document.
Review Objectives
Abstract Conclusion
High performance liquid chromatography (HPLC) cannot be used to

Separate different types of organic pesticides.
Identify the various pigments from a leaf extract.
Determine the caffeine content of coffee samples.
Determine the mercury content of a fish sample.
Any significant technical help received from any individual, source of special
equipment, cultures or other materials is acknowledged in
Abstract Acknowledgement
Results Materials and Methods
The principle of osmotic pressure is used in the
reverse osmosis gel filtration
electroendoosmosis nanofiltration
Gradients of caesium salts are used for separation of
nucleic acids enzymes
proteins cell organells
Page 1 of 2
Transmission electron microscopy is best for high magnification viewing of

internal structure of live, motile cells
internal structure of fixed cells
surface structure of fixed cells
surface membranes of live, motile cells
10) presents the previously published facts and theories in a particular
field.
The result and discussion An abstract
The material and method A review paper
11) Pulse field gel electrophoresis is used to separate
lipids Immunoglobulins
large DNA molecules small DNA molecules
12) type of column chromatography separates proteins on the basis of
molecular weight.
gel filtration chromatography
ion-exchange chromatography
affinity chromatography
isoelectric focusing
13) An abstract of research paper should not exceed words.
500 250
100 50
14) makes the content of book easily accessible to its readers.
An index A table
Introduction References
PART II
Q.2 Discuss the principle, materials used and applications of ultra filtration. 14
Q.3 Describe principle and method of agarose gel electrophoresis of nucleic acids. 14
Q.4 Explain principle, instrumentation, materials and applications of High
Performance Liquid Chromatography.
14
Q.5 Write short answers (any 14
Explain the guidelines for writing a research paper.
Staining of specimens for electron microscopy.
Write on principle and applications of TLC.
Q.6 Write short answers (any 14
Give the guidelines for preparation and submission of proposals to the
funding agencies.
Techniques for Enrichment and isolation of algae
Discuss RNA electrophoresis