M.Sc. (Microbiology) (Semester (CBCS) Examination, 2017
MICROBIOLOGICAL TECHNIQUES AND SCIENTIFIC WRITING
Day Date: Tuesday, 25-04-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
N.B. Part- questions 1 is compulsory.
Attempt any four questions from Part II.
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same
answer booklet only:
PART-I
Q.1 Rewrite the sentences by choosing correct alternative from the
following:
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While preparing manuscript, Introduction section should be presented
in
Present Tense Past Tense
Future Tense None of these
PDF stands for
Portable Document Format Positional Document Format
Portable Disc Format None of these
What is the first step in a scientific investigation?
Asking questions Drawing a conclusion
Making observations Doing research
A research article can be searched by
Author name DOI
Title All of these
Which of the following is a type of paper presentation
Poster Oral
Both None of these
While preparing manuscript, Result section should be presented in

Present Tense Past Tense
Future Tense None of these
Why do scientists communicate their results in written reports?
So other scientists will argue
Because they like to write
To make scientific models
So other scientists can repeat their experiments
Page 1 of 2
is a type of planar chromatography.
Gas Liquid Chromatography Affinity Chromatography
Thin Layer Chromatography HPLC
Which gel is commonly used for the separation of DNA molecules

Agar polyAcrylamide Agarose Ethidium bromide
10) Svedberg is a unit of
Gravitational force Sedimentation rate
Retention time Elution rate
11) Which of the following is used as binding agent in
Calcium Chloride Calcium Sulfate
Cobalt Chloride Magnesium Chloride
12) Ion exchange chromatography is based on
Partition Adsorption
Electrostatic attraction Electrical mobility
13) Deitylaminoethyl cellulose (DEAE-Cellulose) is an example
Anion exchanger Cation Exchanger
Both None of the above
14) In SDS-PAGE, separation is based
Size Shape Molecular Density
PART II
Q.2 Write an essay on SDS-PAGE technique for protein separation 14
Q.3 What is enrichment? Explain various methods involved in enrichment and
isolation of bacteria.
14
Q.4 Briefly describe what is Chromatography? Write in detail Gas Liquid 14
Q.5 Write short answers (any two) 14
Briefly describe 2D Electrophoresis
Making a Oral presentation
Discuss the roles of SDS, TEMED Coomasiae Brilliant Blue in
electrophoresis
Q.6 Write short answers any two. 14
Thin Layer chromatography
Iso-electric focusing
IMRAD system