M.Sc. (Semester (CBCS) Examination Nov/Dec-2018
Microbiology
MICROBIOLOGICAL TECHNIQUES AND SCIENTIFIC WRITING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
Research articles can be searched on Google by
Abstract Letter
Introduction Title
As per basic principles Gas solid chromate graphy is of
chromatography.
Exclusion Ion exchange
Adsorption Absorption
PUBMED /MEDLINE websites are used to
Search literature Sequence matching
Search DNA sequence All of these
Scientific writing mostly use tense.
Future Present
Past Present continuous
is not used as absorbent in column chromatography.
Magnesium Oxide Silica gel
Activated alumina Potassium permanganate
medium is used as selective medium for cultivation of fungi.
Sabouraud's agar Yeast extract agar
Glycerol asparagine agar Nutrient agar
In chromatography different pigments are separated on the basis of
Solubility difference Diffusion
Attraction among particles Capillary action
The long form of EMBL is
European Molecular Biology Laboratory
European Molecular Biotechnology Laboratory
European laboratory of biochemistry
European molecular Laboratory of cell biology
A/An should be viewed as miniversion of the research paper.
Introduction Abstract
Conclusion Discussion
10) Selenite F broth is used for enrichment of organism.
Salmonella Staphylococcus
Bacillus E. Coli
11) Scientific paper is organized in IMRAD method in which stands for
Image Introduction
Investigation Interpretation
Page 2 of 2
SLR-VP-430
12) Research article is published in
Text books Novels
Journals News papers
13) Electrophoresis is not used to separate
RNA DNA
Proteins Amino acids
14) Thickness of absorbent layer in Thin layer chromatography is
0.2 mm 2 mm
0.2 0.2 mili micron
Q.2 Answer the following any four:-
1. Define enrichment.
2. Explain principle of SEM.
3. Define Hela cell line.
4. Enlist media for cultivation of algae.
5. List various tools for research.
08
Answer the following any
1. Explain use of bioinformatics in major research area.
2. Method of staining in Electron microscopy.
3. Solvent system used for separation of amino acids.
06
Q.3 Answer the following Any
1. Explain Electrophoretic separation of lipoprotein.
2. Explain Nano filtration
3. What are components of research paper?
08
Answer the following any 06
1. Quality indicators of scientific journals and impact factor.
2. Enrichment and selective media for isolation of bacteria.
Q.4 Answer the following Any
1. How to prepare a research poster for the conference.
2. Use of different media for cultivation of Protozoa.
3. Explain density gradient centrifugation.
10
Answer the following any
1. Explain what is primary and secondary data.
2. Principles of Ultra centrifugation.
04
Q.5 Answer the following. (Any two) 14
Describe agarose gel electrophoresis for separation of DNA
Write on current good manufacturing practices.
Explain principle and working of electron microscope.