Master of Science I (Microbiology) Examination: Oct Nov 2016
Semester I (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SO-
612
Wednesday
23/11/2016
10.30 AM
to
01.00 PM
Microbiology Techniques
and Scientific Writing SCT 1.1
Instructions: Q.1, Part I is compulsory.
Attempt any four questions from Part II.
Figures to the right indicates full marks
Answer to the Part I and Part II are to be written in same
answer booklet only.
Total Marks: 70
PART I
Q.1 Rewrite the sentence by choosing correct alternative from the following 14
is a type of planar chromatography
Gas Liquid chromatography Affinity Chromatography
Thin Layer Chromatography HPLC
Which of the following is used as binding agent in TLC
Calcium Chloride Calcium Sulfate
Cobalt Chloride Magnesium Chloride
Ion exchange chromatography is base on
Partition Absorption
Electrostatic attraction Electrical mobility
In SDS PAGE, separation is based on
Size Shape
Molecular weight Density
Which gel is commonly used for the separation of DNA molecules

Agar Poly Acrylamide
Agarose Ethidium bromide
Electrophoresis technique for the separation of charged molecules was
developed by
Tiselius Tswett
Ramachandran Sangar
Which of the following is true for an anion
Negatively charged ion that moves towards anode
Positively charged ion that moves towards anode
Negatively charged ion that moves towards cathode
Positively charged ion that moves towards cathode
Which of the following is used to visualize electrophoresed molecules
Silver staining Ethidium bromide
CBB R All of the above
Page 1 of 2
Diethylaminoethyl cellulose (DEAE Cellulose) is an example of

Anion exchanger Cation Exchanger
Both None of the above
10) Electophoretic mobility is affected by
Shape of molecule Size of molecule
Charge on the molecule All of the above
11) While preparing manuscript, Materials Methodssection should be presented
in
Present Tense Past Tense
Future Tense None of these
12) In scientific writing, ideally length of the sentences should be
5 10 words 12 -15 words
35 40 words 30 35 words
13) Most commonly used fontface in slides for oral presentation is
Time New Roman Helvetica
Aerial Courier New
14) DOI stands for
Digital Office Index Digital Object Identifier
Date of Issue None of these
PART II
Answer any four questions from the following
Q.2 Explain in detail Ion-Exchange Chromatography 14
Q.3 What do you mean by scientific Writing? Describe IMRAD system of scientific
writing.
14
Q.4 Explain in detail principle and working of HPLC 14
Q.5 Write short answers (any 14
Draw ray diagram of the Electron Microscope and add a note on working of
SEM
Poster presentation
Importance and significance of documentation in microbiological laboratory.
Q.6 Write short note on any two 14
Thin layer chromatography
Iso electric focusing
Density gradient centrifugation