M.Sc. (Semester (CBCS) Examination Oct/Nov-2017
Microbiology
MICROBIOLOGICAL TECHNIQUES AND SCIENTIFIC WRITING
Day Date: Thursday, 23-11-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Part questions 1 is compulsory.
Attempt any four questions from Part II
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same answer booklet only.
Q.1 Rewrite the sentences by choosing correct given below: 14
Which of the following is used as binding agent in TLC
Calcium Chloride Calcium Sulfate
Cobalt Chloride Magnesium Chloride
Iron exchange chromatography is based
Partition Adsorption
Electrostatic attraction Electrical mobility
Electrophoresis technique for the separation of charged molecules was
developed
Tiselius Tswett
Ramachandran Sanger
Which of the following is used to visualize electrophoresed
Silver staining Ethidium bromide
CBB-R All of the above
Electophoretic mobility is affected
Shape of molecule Size of molecule
Charge on the molecule All of the above
Svedberg is a unit of
Gravitational force Sedimentation rate
Retention time Elution rate
Which gel is commonly used for the separation of DNA
Agar Poly Acrylamide
Agarose Ethidium bromide
In SDS-PAGE, separation is based
Size Shape
Molecular weight Density
Diethy laminoethyl cellulose (DEAE-Cellulose) is an example
Anion exchanger Cation Exchanger
Both None of the above
10) In scientific writing, ideally length of the sentence should
5-10 words 12-15 words
35-40 words 30-35 words
Page 2 of 2
SLR-MQ-582
11) Most commonly used font face in slides for oral presentation is
Times New Roman Helvetica
Aerial Courier New
12) While preparing manuscript, Discussion section should be presented
Present Tense Past Tense
Future Tense None of these
13) What is the first step in a scientific investigation?
Asking questions Drawing a conclusion
Making observations Doing research
14) Summary of the research article
Conclusion Abstract
Discussion None of these
PART II
Answer any four questions from the following.
Q.2 Explain affinity Chromatography with suitable example. 14
Q.3 Write an essay on IMRAD system of scientific writing. 14
Q.4 Explain briefly SDS-PAGE technique for protein separation 14
Q.5 Write short answers Any Two 14
Briefly describe TEM
How to make a Power Point Presentation
Describe Briefly Molecular markers in electrophoresis
Q.6 Write short answers Any Two 14
Paper Chromatography
2D- electrophoresis
Briefly describe Centrifugation