M. Sc. (Biotechnology) (Semester IV) (CBCS) Examination,2017
PLANT BIOTECHNOLOGY
Day Date: Monday, 24-04-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
N.B. Section-I compulsory.
Answer any four question from section-II
SECTION- I
Q.1 Choose the correct alternative given in the bracket. 07
A recombinant DNA molecule is produced by joining together
One mRNA with a DNA segment
One mRNA with a tRNA segment
Two mRNA molecules
Two DNA segment
Which group of enzymes are popularly called "Molecular
stichers"
Restriction Ligases
RNA polymerase DNA polymerase
Expression vectors are those
Produce protein products
Used for genomic libraries
Used for chromosome synthesis
Used for finger printing
Cell without cell wall Is Known as
Protoplast Plasmolysed cell
Both a and b None of the above
Cybrids are
Cytoplasmic hybrids Genomic hybrids
Protoplast None of the above
Meristem culture helps in developing
Hybrid plants Virus free plants
Disease resistant plants Tall plants
Totipotency refers to
The ability of a plant cell to arrest the growth of a plant.
The ability of a plant cell to develop disease in plant
The ability of a plant cell to develop into a complete plant
The ability of a plant cell to develop into a callus
Page 1 of 2
Define the following terms 07
Auxin
Macro-nutrients
Female Gametophyte
Molecular scissor
Secondary Metabolites
Ti Plasmid
Biolistic
SECTION-II
Q.2 Discuses in brief Vector mediated gene transfer in plants with suitable
example?
14
Q.3 What do you mean micro-propagation, explain one with suitable
example?
14
Q.4 Discuss in brief protoplast isolation, culture and its application in plant
biotechnology?
14
Q.5 Answer any Two of the following: 14
Discuses Somaclonal variation with their types.
Shikimate Pathway in plants
Selectable and reporter marker genes.
Q6 write short notes on any Two of the following: 14
Somatic embryogenesis in plants
Vector less gene transformation.
Phytohormones