M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Biotechnology
PLANT BIOTECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section is compulsory. Attempt any 4 question from part- II.
Section I
Q.1
A)Multiple Choice Questions
07
Colchicine is a specific inhibitor of
Spindle fiber
DNA Replication
Protein metabolism
Carbohydrate synthesis
Ti plasmid induceses crown gall disease in
Dicots
Monocots
Grasess
Embryoids
Meristem culture helps in developing
Hybrid plants
Virus free plants
Disease resistant plants
Tall plants
Somatic embryos are
embryos developed from zygote after fertilization
embryos developed from egg without fertilization
embryo like structure developed from the cells of callus
embryo developed by ovules
Totipotency refers to
the ability of a plant cell to arrest the growth of a plant
the ability of a plant cell to develop disease in plant
the ability of a plant cell to develop into a complete plant
the ability of a plant cell to develop into a callus
Deficiency of Magnesium in plants results in
Cholorosis
Necrosis
Both a and b
Elongation of internodes
Gene silencing refers to
Over-expression of gene
No expression of Trans gene
Protein synthesis
All of the above
B)Define the following terms:
07
Phytohormones
35 S promoter
Male Gametophyte
Ti Plasmids
Secondary Metabolites
Micropropagation
Cloning

Section II
Q.2
Discusses in brief about Plant nutrients with their roles in plants.
14
Q.3
Add a brief note on basics of tumor formation in plants.
14
Q.4 Discuss in brief about vector less (Direct gene transfer) transformation in plant?
14
Q.5
Answer any two from the following:
14
a)Application of plant biotechnology.
b)Answer in detail steps involved in Micropropagation.
c)Protoplast Isolation.
Q.6
Write short notes on any two of following:
14
a)Soma-clonal Variation In vitro mutagenesis
b)Shoot tip culture
c)Molecular Farming