M.Sc. (Semester IV) (CBCS) Examination Oct/Nov-2017
Biotechnology
PLANT BIOTECHNOLOGY
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Day Date: Wednesday, 22-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Section-I compulsory.
Answer any four question from section-II
Section I
Q.1 Multiple Choice Question:- 07
Epigenetic variation occurs due to
Tissue culture practices Pre existing variations
Both a and b None of the above
Cybrids are
Cytoplasmic hybrids Genomic hybrids
Protoplast None of the above
Totipotency refers to
The ability of a plant cell to arrest the growth of a plant
The ability of a plant cell to develop disease in plant
The ability of a plant cell to develop into a complete plant
The ability of a plant cell to develop into a callus.
Green Fluorescence protein is a
Selectable marker gene Reporter gene
A gene from animal cell All of the above
Gold Tungsten nano particles are widely used for coating of DNA
fragments in
Electroporation Gene gun
Microinjection All of the above
Protoplasts can be produced from suspension cultures, callus tissues
or intact tissues by enzymatic treatment with
Celluloytic enzyme Pectinolytic enzymes.
Both a b Protease
CaMv is
DNA containing virus
RNA containing virus
Protein containing virus
Both DNA and RNA containing virus
Define the following terms 07
Gene gun
Haploid plant
Embryos
Cholchicine
Viral vectors
Reporter gene
Acclimatization
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SLR-ME-120
Section II
Q.2 Discusses in brief role of Micronutrients and Phyto-hormones in plant growth. 14
Q.3 What do you mean somaclonal variation? Explain in detail mechanism behind
somaclonal variation.
14
Q.4 Discuss in brief about Mechanism of DNA transfer and role of virulence gene
in Agro bacterium mediated gene transfer.
14
Q.5 Answer any Two of the following: 14
Edible Vaccine production
Biotic stress resistance in plants.
CaMV as a cloning vector.
Q.6 Write short notes on any Two of the following: 14
Initiation and maintenance of callus.
Cell and plant tissue culture lab set up.
Plant hormones.