M.Sc. (Semester III) (CBCS) Examination Mar/Apr-2018
Biotechnology
PLANT BIOTECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I is compulsory. Answer any Four questions from section II.
Section I
Q.1

Multiple Choice Question:
07
Epigenetic variation occurs due to
Tissue culture practices
Pre existing variations
Both a and b
None of the above
Cybrids are
Cytoplasmic hybrids
Genomic hybrids
Protoplast
None of the above
Totipotency refers to
the ability of a plant cell to arrest the growth of a plant
the ability of a plant cell to develop disease in plant
the ability of a plant cell to develop into a complete plant
the ability of a plant cell to develop into a callus
Green fluorescence protein is a
Selectable marker gene
Reporter gene
A gene from animal cell
All of the above
Gold/Tungsten nano particles are widely used for coating of DNA fragments in
Electroporation
Gene gun
Micro injection
All of the above
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
Celluloytic enzyme
Pectinolytic enzymes
Both a and b
Protease
CaMv is
DNA containing virus
RNA containing virus
Protein containing virus
Both DNA and RNA containing virus

Define the following terms:
07
Gene gun
Haploid plant
Embryos
Cholchicine
Viral vectors
Reporter gene
Acclimatization
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SLR-UE-95
Section II
Q.2
Discuss in brief role of Micronutrients and Phyto-hormones in plant growth?
14
Q.3
What do you mean somaclonal variation? Explain in detail mechanism behind somaclonal variation.
14
Q4
Discuss in brief about Mechanism of DNA transfer and role of Virulence gene in Agro bacterium mediated gene transfer?
14
Q5
Answer any two from the following
14
Edible Vaccine production
Biotic stress resistance in plants
CaMv as a cloning vector.
Q.6
Write short notes on any Two of the following:
14
Initiation and maintenance of callus
Cell and plant tissue culture lab set up
Plant hormones