M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Biotechnology
PLANT BIOTECHNOLOGY
Day Date: Tuesday, 21-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: All questions of Section I are compulsory.
Answer any Four questions from section II.
All question carry equal marks.
Draw neat and labeled diagrams wherever necessary.
Section I
Q.1 Multiple Choice Question: 07
Colchicine is a specific inhibitor of
Spindle fiber DNA Replication
Protein Metabolism Carbohydrate synthesis
2 Ti plasmid induceses crown gall disease in
Dicots Monocots
Grasess Embryoids
3 Meristem culture helps in developing
Hybrid plants Virus free plants
Disease resistant plants Tall plants
4 Somatic Embryos are
Embryos developed from zygote after fertilization
Embryos developed from egg without fertilization
Embryo like structure developed from the cells of callus
Embryo developed by ovules
5 Totipotency refers to
The ability of a plant cell to arrest the growth of a plant
The ability of a plant cell to develop disease in plant
The ability of a plant cell to develop into a complete plant
The ability of a plant cell to develop into a callus
6 Deficiency of magnesium in plants results in
Cholorosis Necrosis
Both a and b Elongation of internodes
7 Gene silencing refers to
Over-expression of gene No expression of Trans gene
Protein synthesis All of the above
Page 2 of 2
SLR-ME-108
Define the following terms: 07
Phytohormones
35 S promoter
Male Gametophyte
Ti Plasmids
Secondary Metabolites
Microprpogating
Cloning
Section II
Q.2 Discusses in brief about Plant nutrients with their roles in plants. 14
Q.3 Add a brief note on Basics of tumor formation in plants. 14
Q4 Discuss in brief about vector less (Direct gene transfer) transformation in plant? 14
Q5 Answer any two from the following 14
Application of plant biotechnology
Answer in detail steps involved in Micropropogation
Protoplast Isolation
Q.6 Write short notes on any Two of the following: 14
Soma-clonal Variation/ In vitro mutagenesis
Shoot tip culture
Molecular Farming