1. One of the following is NOT a 00-6 poly unsaturated fatty ,acid.

Linoleic acid

y-linolenic acid

Palmitoleic acid

Dihomo-y-linolenic acid

2. A competitive inhibitor of an enzyme

increases Km without affecting Vmax

decreases Km without affecting Vmax

increases Vmax without affecting Km

decreases Vmax without affecting KIn

3. An inborn error, maple syrup urine disease is due to deficiency of the enzyme

sovaleryl-CoA-hydrogenase

Phenylalnine hydroxylase

Adenosyl transferase

a-Ketoacid decarboxylase

4. Various transcripts are located in distinct regions of developing embryo which can be best demonstrated by

Northern hybridization

Real-time PCR

In situ hybridization

Western blotting

5. How much MgCh is required to make one litre of 0.1 molar MgCh solution?

0.95

9.5g

95g

950 g

6. The pH of blood is 7.4 when the ratio between H2C03 and NaHC03 is

1:10

1:20

1:30

1:15

7. Fatty acids can be transported into and out of cell membrane by

Active transport

Diffusion

Osmosis

Facilitated transport

8. Which one of the following confers passive immunity?

Hepatitis B vaccine

Hepatitis B immunoglobulin

MMR vaccine

Infection

9. Cystic fibrosis is caused by a recessive allele. A child has CF, even though neither of the parents shows CF symptoms. If this couple has another child, what is the probability he or sh'e will NOT have CF?

1/4

2/4

3/4

1/3

10. Which one of the following animals use extensively, anal gland secretion for territory marking?

Earthwonn

Insects

Fishes

Big cats

11. Wheat germ agglutinin binds strongly to

Slu-face glycoproteins on activated T-and B-cells.

Surface lipoproteins on activated T­and B-cells.

Surface glycoproteins on resting T­cells.

Surface lipoproteins on resting T­cells.

12. Nude mice are deficient in

Macrophages

Mature B cells

NK Cells

Mature T cells

13. Alpha-fetoprotein is an oncofetal antigen which is a diagnostic marker for

Colon cancer

Liver cancer

Chronic myeloid leukemia

Breast cancer

14. How are trypanosomes able to evade the host immune system?

They have an antiphagocytic capsule

They are obligate intracellular parasites

They continuously alter their surface antigens

They produce enzymes that digest antibodies

15. Which one of the following ions is critically associated with meiotic maturation of the mammalian oocyte?

Ca2

Zn+



Mg2+

16. The reaction mixture for the assay of glutamate dehydrogenase contains 100 f.ll of 0.1 M NAD+. The quantity of NAD+ added is

1 umoles

10 umoles

100 umoles

1000 umoles

17. One of the following is NOT associated with gluconeogenesis.

G6PD

Glucose 6-phosphatase

FDPase

PEPCK

18. Activation of phospholipase C causes all of the following EXCEPT

Production of diacyl glycerol

Release of Ca2 from intracellular
stores

Cleavage of membrane -bound PIP3

Dephosphorylation of proteins

19. Which one of the following virus is NOT used in gene therapy?

Retrovirus

Adenovirus

Adeno-associated virus

Polyomavirus

20. The aminoglycosides are a very active group of antibacterial agents, particularly against Gram-negative Bacilli. Identify their mode of action from the list.

Disruption of cytoplasmic membrane function

Inhibition of bacterial cell wall synthesis

Inhibition of protein synthesis

Inhibition of bacterial DNA gyrase

21. Antibody-dependent cytotoxicity is associated with

Type I Hypersensitivity

Type II Hypersensitivity

Type III Hypersensitivity

Type IV Hypersensitivity

22. Potent environmental estrogen which was banned by FDA as drug for promoting clear cell carcinoma in 1971 is

Nonylphenol

Atrazine

Diethylstilbestrol

Estradiol

23. Which one of the following signal cascades depends on cell-cell interactions?


Ras signaling

Jak-Stat signaling

Notch signaling

NO signaling

24. Neoteny is seen in

Frogs

Salamanders

Snakes

Crocodiles

25. Epithelial mesenchymal interaction required for the development and differentiation of liver and pancreas

Ectoderm and Mesoderm

Ectoderm and Endoderm

Endoderm and Mesoderm

Endoderm and neural crest

26. Which one of the following animal uses Osphrad!um to test the purity of water?

Pita

Asterias

Stichodactyla

Hydra

27. GABA (gama amino butyric acid) is

post-synaptic excitatory transmitter

post-synaptic inhibitory transmitter

activator of glia-cell function

inhibitor of glia-cell function

28. Which one of the following hormones is NOT produced by adrenal cortex?

Aldosterone

Ponasterone

Cortisol

Testosterone

29. In mammalian DNA, transcription regulatory regions containing CpG islands are inactivated by

Myristylation

Methylation

Phosphorylation

Acetylation

30. A 'fastidious' microorganisms is the one that

Shows mediuln rapid growth in minimal

Can utilize only glucose as a carbon and energy source

Requires growth specialized nutr ients for growth

Cannot be cultured in artificial media

31. A person heterozygous for an autosomal dominant 'disorder marries an individual who is homozygous recessive and have children, which statement is CORRECT?

All of their children will be carriers

None oftheir children will have the disorder

All of their children will have the disorder

Half of their children will have the disorder

32. A mutation is most likely to alter the three-dimensional conformation of a protein if

There is a substitution of a hydrophobic amino acid for a hydrophilic amino acid

Valine is substituted for leucine

It changes the amino acid at the amino-terminus

It places proline in the middle of an alpha-helix.

33. Which one of the following statements about the synthesis of carbamoyl phosphate by carbamoyl phosphate synthase-I is INCORRECT?

The enzyme catalyzes the rate­limiting reaction in the urea cycle.

The reaction is reversible

The reaction is allosterically activated by N-acetyl glutamate

The reaction requires two high-energy phosphates for each carbamoyl phosphate molecule to be synthesized

34. One of the following enzymes is involved in ammonia detoxification.

Glutaminase

Lactate dehydrogenase

Glutamate dehydrogenase

Malate dehydrogenase

35. Oxidation of which substance in the body yields most calories?

Lipid

Glucose

Glycogen

Protein

36. Riboflavin is a coenzyme in the reaction catalyzed by the enzyme

Acyl CoA synthetase

Enoyl CoA dehydrogenase

Acyl CoA dehydrogenase

3-hydroxy acyl CoA dehydrogenase

37. SDS-PAGE of the secretory IgA immunoglobulin shows number of bands.

1

2

3

4

38. The 'Origin of Life on Earth' was proposed by

Alexander Ivanovich Oparin

Charles Darwin

Friedrich Miescher

Oswald Avery

39. Which one of the following statements is NOT correct?

Hypoparathyroidism results in hypocalcemia.

Postmenopausal women normally suffer from osteoporosis.

FSH deficiency leads to hypogonadism associated with failure of germ cell maturation

Secretin regulates pancreatic hormone secretion

40. Which one of the following represents the most reduced form of carbon?

R-CH3

R-COOH

R-CHO

R-CH20H

41. Morphallactic pattern of regeneration is seen in

Wall lizard tail

Salamander fore limb

Frog tadpole tail

Hydra body

42. Which one of the following statements is NOT true?

Fosmid clones are similar in size as cosmids

Fosmid clones contain replicons derived from F factor

BAC clones are like Fosmids

HMW DNA is the source for generating Fosmids

43. The activity of kinins is modulated by

Prostaglandins



Increased cAMP level

Increased cGMP level

44. Following recombinant protein is commercially produced from mammalian cell culture?

Insulin

Tissue plasminogen activator

Hepatitis B

Taq polymerase

45. An important function of vitamin A is

To act enzymes as coenzyme for a few coenzymes

To maintain the integrity of epithelial tissue

To play an integral role in protein synthesis

To prevent hemorrhages

46. The neurologic disturbances seen in Niemann-Pick disease are associated with the accumulation in the central nervous tissue

Phosphatidyl choline

Phosphatidyl serine

Sphingomyelin

Gangliosides

47. Bacteriophage consists of

Only proteins

Nucleoproteins

Only DNA

OnlyRNA

48. In which pair of diseases, both are caused by viruses?

Measles rabies

Syphilis AIDS

Tetanus Typhoid

Whooping cough sleeping sickness

49. Which one of the following is a cell-coded protein formed in response to infection with most animal viruses?

Histone

Antibody

Antigen

Interferon

50. Which one of the following co-enzyme is also called as co-substrate of enzyme?

NAD+

ATP

FADH2

Heme

51. Membrane carrier proteins differ from membrane channel proteins by which of the following characteristics?

Carrier proteins are glycoproteins, while channel proteins are lipoproteins.

Carrier proteins can mediate active transport, while channel proteins cannot.

Carrier proteins do not bind to the material transported, while channel proteins do.

Carrier proteins transport molecules down their electrochemical gradient, while channel proteins transport molecules against their electrochemical gradient.

52. The unit of the molar extinction coefficient is

L. mole-1. cm-1

L. mole. cm-1

L. mole-1. cm

L-1 . mole-1. cm­1

53. Propagation of a regenerative action potential along an axon can be accelerated by which of the following?

A decrease in the transmembrane resistance

A decrease in the axoplasmic resistance

Reduced myelin wrapping

Shortened intemodallengths

54. Which of the following is true about a circular double-stranded DNA containing 210/0 adenosine?

It has 21 guanosine

It has 580/0 guanosine

It has 29% guanosine

It has 42% guanosine

55. Which among the following vaccines is NOT an attenuated whole organism?

Salk

BCG

Sabin

Tetanus

56. Which one of the following pairs of structures depicts stereoisomers according to conventional rules of projection?

<img src='./qimages/2-56A.jpg'>

<img src='./qimages/2-56B.jpg'>

<img src='./qimages/2-56C.jpg'>

<img src='./qimages/2-56D.jpg'>

57. Elevated levels of circulating glucagon are associated with which one of the following?

Increased activity of phosphofructokinase-2

Decreased activity of fructose 2,6­bisphosphatase

Decreased activity of fructose 1,6­bisphosphatase

Increased activity of 1,6­bisphosphatase

58. DNA polymorphism in one of the following led to the development of DNA fingerprinting as a forensic tool.

VNTR

Intronic sequencing

Enhancer elements

Heteroduplex DNA

59. Inhibin hormone is comnionly produced by following endocrine glands.

Pituitary

Pancreas

Gonads

Parathyroid

60. Which glycosaminoglycan does NOT contain uronic acid?

Dermatan sulphate

Keratan sulphate

Chondroitin sulphate

Heparan sulphate

61. All of the following statements regarding ketone bodies are true EXCEPT

They may result from starvation

They include acetoacetic acid and acetone

They may be excreted in urine

They are formed in kidneys

62. Which of the following is NOT mediated by lipid component of the membrane?

Channel formation

Barrier to passage of water soluble substances

Fluidity of membrane

Structural boundry

63. Peptide present in egg jelly of sea urchin, which plays important chemotactic role during fertilization, is

Fertilizin

Resact

Bindin

Activin

64. Congenital adrenal hyperplasia is known to cause

Female pseudohennaphorditism

Male pseudohermaphorditism

Turner's syndrome

Klinfelter's syndrome

65. Which one of the following is NOT a bHLH family protein?

MyoD

Pax1

Myf5

c-Myc

66. Protein critical for establishment of anterior-posterior polarity in Drosophila embryo is

beta-catenin

Kruppel

Bicoid

Fushi tarazu

67. A fatty acid which is NOT synthesized in human body and has to be supplied in the diet

Palmitic acid

Oleic acid

Stearic acid

Linoleic acid

68. Lipases can act between pH range of

2.5-4

3.5-5

5-7

4-5

69. Which one of the following is NOT required for the RecA-dependent recombination between two DNA molecules?

Strand migration

Ligation

Nuclease digestion

Mismatch repair

70. An E. coli strain lacking DNA polymerase I would be deficient in DNA

Repair

Methylation

Transcription

Degradation

71. Which of the following types of information CANNOT be determined from the traditional Northern blotting technique?

The size of an mRNA species

The half-life of an mRNA species

The relative levels of an mRNA species in different tissues

The amino acid sequence of the protein coded by an mRNA species

72. The most common form of speciation is

Sympatry

Allopatry

Parapatry

Antipatry

73. Kinesis, animal's response to stimulus, is

Directional

Uni-directional

Multi-directional

Non-directional

74. Various oncogenes may encode all of the following EXCEPT

Growth factors

Kinases

Receptors

Tumor suppressor proteins

75. Milk is deficient of which mineral?

Calcium

Iron

Potassium

Sodium For rough work