M.Sc. (Semester (CBCS) Examination Nov/Dec-2018
Microbiology
RECENT TRENDS IN VIROLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagram wherever necessary.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
In lysogeny, delayed early gene products cII and cIII are necessary for RNA
polymerase to initiate transcription at the promoter
PL PR
PRM PRE
As of 2017, orders, 131 families, 46 subfamilies, 803 genera and
4,853 species of viruses have been defined by the ICTV.
11 9
7 8
Antiviral substance produced in human body is
Antibody Immunogen
Antigen Interferon
bacteriophage contain single stranded RNA.
6 M13
PM2 MS2
Classification of viruses is based on the method of viral mRNA
synthesis.
ICTV LHT
Baltimore Holmes
Viral genomes often contain short sequence or set of sequences
that directs encapsidation.
Terminal signal Packaging signal
Coding signal Genomic signal
The phage ØX-174 genome penetrates the host cell with the help of pilot
protein which is product of
K gene M gene
J gene H gene
The term viroid was discovered by
Baltimore Diener
Puschner Boin
In Hepatitis C virus is genome.
ds DNA Segmented DNA
RNA ssDNA
10) virus contain double stranded RNA.
Reovirus Bunya virus
Calcivirus Rhabdovirus
Page 2 of 2
SLR-VP-429
11) In Pock assay, viral dilution is inoculated onto the surface of
Yolk sac Amniotic cavity
Chorioallantoic membrane Allantoic cavity
12) TMV virus contains genome.
ds DNA ssRNA
ds RNA ssDNA
13) Viroid genomes are extremely small in size, only about nucleotides.
30 300
100 600
14) A type of cell culture that can reproduce for an extended number of
generations and is used to support viral replication is a
Continuous cell line Cell strain
Diploid fibroblast cell Primary cell culture
Q.2 Define and explain any four of the following:-
1. Repression
2. ICTV
3. Cell transformation
4. Excision
5. Cell transformation
08
Write short note any two of the following:-
1. Satellite viruses
2. Prions
3. Latex Droplet method
06
Q.3 Answer the following Any
1. Tissue culture for Animal Virus Cultivation.
2. History of Viruses
3. Brief outline of classification of viruses.
08
Answer the following any one
1. Briefly describe Viroids.
2. Emerging viral infections.
06
Q.4 Answer the following Any
1. Describe lysogeny.
2. Give an account of Interferon
3. Give brief account of Infectivity assays.
10
Answer the following any one
1. Give brief account of T4 phage lytic cycle.
2. Describe Oncogenesis.
04
Q.5 Answer the following. (any two) 14
Discuss genetic analysis of Viruses by Classical Genetic methods.
Describe briefly the Emerging viruses with examples.
Describe in detail Plant Viruses.