1. In the year 2005, the "Golden Rice-2" was developed using transgenic approaches which contain 23 fold more as compared to original golden rice

Vitamin A

Zinc content

Vitamin B

Iron content

2. Which vitamin is called as 'beauty vitamin'

Biotin

Retinol

Tochoferol

Calciferol

3. Gas chromatography is used for metabolomics study for separating and analyzing compounds that can be vaporized without decomposition. Which of the following is the most suitable gas to use as a carrier gas in a gas chromatogram?

Methane

Hexane

Ammonia

Helium

4. In C4 plants, which enzyme performs the critical reaction of converting carbon dioxide to 3-phosphoglycerate?

RuBP carboxylase

PEP carboxylase

RuBP activase

PEP decarboxylase

5. Which one of the following is not a blue light photoreceptor in plants?

Phototropin

Cryptochrome

Zeitlupe

UVR-8

6. Which one of the following is not a peptide hormone in plants?

Phytosulfokine

Strigolactone

Systemin

Stomagen

7. The typical genetic ratio expected for a population segregating for a resistant trait controlled by digenic complementary recessive genes



9:7

15: 1



8. "Hidden epitopes" are classified as

Metatopes

Neotopes

Cryptotopes

Neutralizing epitopes

9. Seed dispersal by ants is called as

Elaiosomochory

Blastochory

Anemochory

Meterochory

10. These are called as Amphibians of Plant Kingdom

Pteridophytes

Gymnosperms

Bryophytes

Fungi

11. Gamma Knife and Cyber knife are two systems that are used in the treatment of

Cancer

HIV and AIDS

Arthritis

Eye Cataracts

12. Which of the following is a target signal for proteins targeted to peroxisomes









13. Which of the following is a site specific endonuclease in T-DNA transfer?

VirDI

(b)VirBII

(c)VirE2

(d)VirD2

14. Which is the name associated with the development of 'gene gun' technique in transformation?

Boynton

Pal Maliga

J.e. Stanford

J. Schell

15. Microcephaly is associated with

SARS virus

ZIKA virus

EBOLA virus

HANTA virus

16. 'Gossypol' acts as an inhibitor for several dehydrogenase enzymes is derived from

Groundnut

Chestnut

Cotton

Mexican Chillies

17. Organisms that live inside rocks or in pores between mineral grains are called as

Endoliths

Extremophiles

Psychrophiles

Meshophiles

18. Global warming is associated with the increase in the atmospheric C02 concentration, what is the present global atmospheric C02 concentration.

358 ppm

404 ppm

453 ppm

507 ppm

19. A disease cycle in which a pathogen that takes several years to initiate new infection is called

Monocyclic cycle

Polycyclic

Polyetic cycle

Dicyclic

20. The herbicide phosphinothricin targets the enzyme

Acetolactatesynthase

5-enol pyn.ivyl 'shikimate-3-phosphate synthase

Itrilase

Lutamine synthetase

21. Which of the following photosynthesis bacteria have both PS I PS II?

Purple Sulphur bacteria

Cyanobacteria

Purple non Sulphur bacteria

Green Sulphur bacteria

22. Hypsochromic shift is nothing but change in spectral position to

Higher wavelength

Less energy frequency

Shorter wavelength

Shorter wavelength and lower frequency

23. Anthracyclines are a class of antibiotics, produced from

Strptomyces persutus

Bacillus megaterium

Pseudomonas campestris

Podophyllum peltatum

24. Commensalism is a biological relationship between individuals of two species in wHich

Both the species compete for space and food

One species obtains food or other benefits from the other without either harming or benefiting the latter

One species obtains food or other benefits from the other by harming the latter

Both the species are of the concept of "give and take"

25. LOD scores are used in genetics

For linkage analysis in human or animal or plant populations

To study epistatic interactions between two genetic loci

To estimate the frequency of double cross overs

To estimate the penetrance of allele

26. Which of the following proteins are associated with male sterility restoration in plants

PPR proteins

F-Box proteins

VIP proteins

TATA box proteins.

27. Paclitaxel, the most well-known natural-source cancer drug, is derived from the bark of

Vinca rosea

Gloriosa superba

Pacific yew tree

Strychnos

28. A population of a host in which all individuals have a particular character of resistance in common is called

Biotype

Pathovar

Pathodeme

Biotroph

29. Which one of the following plant hormone is not derived from carotenoid degradation?

Abscisic acid

Phaseic acid

Brassinosteroid

Strigolactone

30. The isotope of Hydrogen usually used in radioactive labeling of nucleic acids is

H2

¹H

²H

³H

31. Plant with robust root system penetrating into deeper ground water is

Phreatophyte

Epiphyte

Sporophyte

Cryptophyte

32. Kaolinite and quartz enriched soils are

Ferralsol

Vetrisol

Podsol

Lixisol

33. One of the amino acid is involved in phosphorylation by post translation modification

(a)Valine

Methionine

Cysteine

Threonine

34. Instrument used for measuring the height of a standing tree

Hypsometer

Lysimeter

Stratometer

Manometer

35. This scientist is associated with the discovery of Potato Spindle tuber viroid in 1971.

Brakke

T. O. Diener

William Stanley

Renatto Dalbecco

36. The vernalization treatment leading to flower development is sensed by

Leaf

Shoot apex

Stem

Root

37. Which of the following protein is considered as florigen in the plants?

Constans

Gigantea

FT

Wus

38. Norman Ernest Borlaug, who has been called "the father of the Green Revolution" has been awarded Noble Peace Prize in the year

1968

1970

1972

1974

39. Phytosiderophores are involved in transport offoHowing mineral ion from soil to root cells In dicot plants.

Silicon

Boron

Iron

Potassium

40. Curstose, fruticose and foliose are varieties of

Rusts and smuts

Yeasts

Lichens

Mycorrhizae

41. Which of the following is the best host E. coli strain for expressing a protein that must contain disulphide bonds for it to function properly?

(a)BL21(DE3) Origami

BL21 pLysS

BL21 Rosetta

BL21 Star

42. A common photoreceptor between plant and animals is

Phytochrome

Rhodopsin

Phototropin

Cryptochrome

43. DNA profiling technique to demonstrate the similarity between different plant species with a reference to some specific protein coding DNA sequences is called

Plant profiling

Phyto blot

Garden blot

Southern blot

44. Arabidopsis and rice have diploid chromosome number of 10 and 24, respectively. Assuming no crossing over taking place, genetic variation among F2 individuals in a genetic cross is likely to be.

Same in both species but not zero

More in Arabidopsis

More in rice

Zero in both the species

45. In bacterial promoters, which of the following describes the 'Pribnow-Schaller

The untranslated region.

The -10 box

The -35 box

The termination sequence

46. When the bark of a tree is removed in a circular fashion all around near its base, it gradually dries up and dies because

Water from soil cannot rise to aerial parts

Roots are starved of energy

Tree is infected by soil microbes

Roots do not receive oxygen for respiration

47. What is the amount of energy released at the end of glycolysislKrebs cycle?

(a)678,000 calories

687,000 Calories

597,000 Calories

569,000 Calories

48. Bacterial endospores have very specialized proteins which protect the spores from DNA damage, these are called as

Small acid soluble proteins (SASP)

DNA recovery proteins

Endospore specific soluble proteins (ESSP)

Spore specific mega proteins (SSMP)

49. Which type of database is 'FlyBase'

Literature database

Bimolecular database

Model organism database

Biodiversity database

50. A bacterial cell with the fertility factor, F integrated into its chromosome is referred as

Excongujant

Merozygote

High frequency of recombination

Resistance transfer factor

51. The mobile phase in reverse phase chromatography is

Non polar organic solvent

Basic buffer

Acidic buffer

Polar organic solvent

52. Lipid-a stimulates the synthesis of one of the following factors

Transforming growth Factor

Platelet-derived growth factor (PDGF)

Epidermal growth factor

Tumor necrosis factor

53. Which of the following is a sensor of phenolics in Agrobacterium?

Vir D1

Vir C2

Vir A

Vir B

54. What are apocarotenoids?

These are terpenoids with C40 carbon

These are terpenoids with >C40 carbon

These are terpenoids with <C40 carbon

These are terpenoids with C40 carbon and are conjugated with glucose.

55. Algae and other submerged plants in water float during day time and sink at night, because

They come up to enjoy some time

They lose weight at night

They become buoyant due to accumulation of 02 as a result of photosynthesis

They become light due to food material accumulation

56. Proteasomes destroy proteins by unfolding them and chopping them into small fragments. What kinds of proteins are recognized by proteasomes?

(a)Proteasomes recognize ubiquitinylated proteins.

Proteasomes recognize denatured proteins.

Proteasomes recognize proteins with basic or aromatic amino acids at the N-terminus.

Proteasomes recognize viral proteins.

57. Phenol used in DNA extraction

(a)Precipitates DNA and leave proteins' in aqueous solution

Precipitates RNA-protein complex and leave DNA in aqueous solution

Precipitates cell debris and leave nucleic acids-protein complex in aqueous solution

Precipitates proteins and leave nucleic acids in aqueous solution

58. Which of the following statements is true of RNA interference?

(a)RNA interference is a normal way for organisms to regulate gene expression.

RNA interference is a mechanism for combating virus infection in plants.

Both A and B

RNA interference is already used therapeutically for many disorders

59. Cellulose is composed from D-glucose units, which condense through

alpha(l--->4)-glycosidicbonds

bonds

bonds

alpha(l--->6)-glycosidicbonds

60. The coding region of a gene is 105 nucleotides long, including both start and stop codons, which of the following would be the most likely to effect of a single nucleotide deletion at position 79 in the coding region?

(a)Only the active site would be affected

There would be no effect on the polypeptide

The entire amino acid sequence of the polypeptide would change

There would be changes in only the last 9 amino acids

61. What is a 'proteotypic' peptide?

post-translationally modified peptide

A stable isotope-containing peptide

A peptide which is unique to a specific protein

A peptide which is typical of all other peptides

62. Which among the following statements is not correct for the differences between bacterial photosynthesis vs plant photosynthesis?

Bacteria lack definite chloroplast while in plants photosynthesis happen in chloroplast

Photosynthesis takes place above 700 nm in bacteria, while in plants it occurs between 400 to 700 nm

The C02 reductant in bacteria is NADH while in plants the reductant is NADPH+H+

Non-cyclic photophosphorylation is dominant in bacteria, while cyclic photophosphorylation in plants

63. Match the following

i. Nicotine A. Grass

ii. Cynogenic glucosinolates B. Terpenoid

iii Silica cells C. Cysteine-rich protein

iv. Limonene D. Alkaloid

v. Defensin E. Hydrogen cyanide



i ii iii iv v

i ii iii iv v

i ii iii iv v

i iii iv v

64. "Lantibiotics" are

A group of probiotics which will promote the growth of many bacteria

A group of prebiotics which will promote the growth of many bacteria

A group of antibiotics which are polycyclic peptides

A group of toxins which are produced by micro organisms

65. Which of the following statement is CORRECT on plant adaptation?

Plants adapted to cold environment have higher ratio of 'unsaturated to Saturated' fatty acids in their membrane compared to those adapted to hot Environment.

Plants adapted to cold environment have lower ratio of 'unsaturated to saturated' fatty acids in their membrane compared to those adapted to hot environment.

Plants adapted to cold environment have same ratio of 'unsaturated to saturated fatty acids in their membrane compared to those adapted to hot environment.

Plants do not have any unsaturated fatty acids in the membrane.

66. Which of the following statements about isoelectric focusing is correct?

(a)Proteins separated by isoelectric focusing cannot be tested for biological activity.

Proteins separated by isoelectric focusing can be tested for biological activity.

The separation of proteins by isoelectric focusing is only based on charge.

The separation of proteins by isoelectric focusing is only based on size.

67. For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed

(a)Affinity chromatography followed by size exclusion chromatography

AC only

AC followed by ion-exchange followed by SEC

AC followed by lEX, followed by hydrophobic interaction and then SEC

68. Which among the following is not a correct statement for terpenoids

Terpenoids are polymers of isoprenoids

Isopentanyl pyrophosphate is the processor for terpenoids

Biogenesis ofterpenoids occurs only in plants and prokaryotes

Steroids, hopanoids and quinones are a class ofterpenoids

69. Many plants inhibit different types of pathogens and herbivores using special chemical known as secondary metabolites. When an insect larva consumes this secondary metabolites containing plant tissue, this secondary metabolite incorporated into the insect's protein in some of the places where the mRNA codes for arginine, because the enzyme that charges the tRNA specific for arginine fails to discriminate accurately between the two amino acids. This secondary metabolites is an amino acid that is not found in protein, but is very similar to the amino acid arginine. The structure of this secondary metabolite, however, is different enough from that of arginine, that some of the resulting proteins end up with a modified tertiary structure, and hence reduced biological activity. These defects in protein structure and function lead to the developmental abnormalities that kill the insect. What is the name of this secondary metabolite?

Phytoalexin

Canavanine

Gallotannin

Nicotine

70. Bacterial cell lysis by lysozyme is due to the

Hydrolysis of alpha-1 ,4-glycosidic bonds between the N-acetyl glucosamine and N-acetylmuramic acid

Inhibition of cell wall synthesis

Hydrolysis of pentapeptide bridges

Hydrolysis of ß-l,4-glcosidic bonds between the N-acetyl glucosamine and N-acetylmuramic acid

71. The word "anammox" refers to

A group of micro organisms which are involved in ammonification

A chemical process which involves ammonification

A group of anaerobic micro organism which are involved in ammonia oxidation

A group of anoxygenic micro organisms which are involved in ammonia oxidation

72. In molecular biology and genomics different types of vectors are used for gene cloning or cloning of large eukaryotic DNA. The ideal feature of the cloning vectors should be small in size and have an antibiotic resistance gene as selectable marker. The BAC, PAC and YACs are different types of cloning vectors used for eukaryotic DNA cloning. What is the correct size and selectable markers of these 3 vectors?

BAC 7.4Kb Chloramphenicol resistance gene; PAC l8.75Kb Kanamycin resistance gene; YAC 11.45Kb Ampicillin resistance gene

BAC 7.4Kb Ampicillin resistance gene; PAC l8.75Kb Chloramphenicol resistance gene; YAC 11.45Kb Kanamycin resistance gene

YAC 7.4Kb Chloramphenicol resistance gene; BAC 18.75Kb Kanamycin resistance gene; PAC 11.45Kb Ampicillin resistance gene

BAC 7.4Kb Ampicillin resistance gene; PAC Il.45Kb Kanamycin resistance gene; YAC l8.75Kb Chloramphenicol resistance gene

73. The steps in setting up a Pichia pastoris expression system are listed below. Work out which of the options A-D lists the correct sequence of steps.

A. Transform competent Pichia cells

B. Add the coding sequence for an affinity purification tag to the cDNA for your 'protein of interest'

C. Determine methanol utilization phenotype

D. Screen growth and induction conditions

E. Linearize the expression vector by restriction digestion

F. Sub-clone the cDNA for your 'protein of interest' into the expression vector

G. Select transformed Pichia cells by loss of auxotrophy or antibiotic resistance

H. Screen for 'jackpot clones'









74. Several types of DNA sequencing technology are available now-a-days which are used for whole genome sequencing of an organism. One of such technology is Next Generation Sequencing (NGS). Which of the following technique best describe the NGS?

GC-MS, LC-MS and NMR

Sequence Tag Site and Expressed Sequence Tag

Roche's 454, Illumina and ABI's SOLiD

Whole genome shotgun and Clone-by-Clone approach

75. Pan-genome or "supra-genome" includes

"Core genome" containing genes present in all strains, a "dispensable genome" containing genes present in two or more strains, and finally "unique genes" specific to single strains

Only "core genome" containing genes present in all strains

"Core genome" containing genes present in all strains and "dispensable genome" specific to single strains

"Core genome" containing genes present in all strains and "unique genes" specific to single strains