EXAMINATION -2014 Ph.D. Plant Sciences
Time: 2 hours Maximum Marks: 75
.HALL TICKET NO.

INSTRUCTIONS Please read carefully before answering the questions:
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Enter your Hall Ticket number both on the top ofthis page and on the OMR answer sheet.

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Answers are to be marked only on the OMRanswer sheet following the instructions provided there upon.

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Hand over the OMR answer sheet to the Invigilator before leaving the examination hall.

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The question paper contains 75 questions. Part-A: Question Nos. 1­25 and Part-B: Questions Nos. 26-75 of multiple-choice printed in 15 pages, including this page. One OMR answer sheet is provided separately. Please check.

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The marks obtained in Part-A will be used for resolving the tie cases.

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Each question carries one mark.

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There is Negative marking for wrong answers, in Parts A and B. For each wrong answer, 0.33 mark will be deducted.

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Calculators and mobile phones are NOT allowed.


PART-A


1. Identify among the following the enzyme that operates only in glyoxylate cycle,
but not during respiratory reactions

A. Citrate synthase
B. Malate dehydroganse
C. Malate synthase
D. Fumarase
2. Catharanthus roseus, which is a source of an alkaloid, Vinblastine, belongs to the family
A. Annonaceae
B. Apocynaceae
C. Acanthaceae
D. Euphorbiaceae
3. Cytochrome b6-f complex occurs in
A. Mitochondria only
B. Chloroplasts only
c.
Both mitochondria and chloroplasts

D.
Neither mitochondria nor chloroplasts


4. What is reverse methanogenesis?
A. Oxidation ofmethane to carbon dioxide
B. Oxidation of methane to methanol .
C. Conversion ofmethane to methyl halides
D. Conversion of methane to biomass
5. Why is an RNA primer necessary for DNA replication?
A. The RNA primer is necessary for the activity of Helicase
B. Only RNA primer can detect the template strand
C. DNA polymerase can only add nucleotides to RNA molecules
D. DNA polymerase can only add nucleotides to an existing strand

6. Which among the following does not belong to Proteobacteria
A. Neisseria
B. Vibrio
C. Helicobacter.
D. Enterococcus
7. 'Operational taxonomic unit' refers to
A. Taxonomic units, which are valid in the nomenclature
B. Taxa whose phylogenetic level is not known
C. Taxa whose phylogentic level is known
D. Taxa which are validly described
8. Diaminopimelate pathway leads to the synthesis of
A. Glutamate
B. Lysine
c.
Leucine

D.
Isoleucine


9. What is the original source of all variations in heritable traits?
A. Natural selection
B. Artificial selection
C. Mutation
D. Adaptation
10. What is an adaptive trait?
A. Any trait that can be passed on to the next generation
B. Any trait that remains constant over time
C. Any trait that helps an organism survive and reproduce
D. Any trait that can not be changed by mutation
11. What is the function ofthe control group in an experiment?
A. To serve as a source of backup subjects
B. To increase the number ofparticipants
C. To serve as a standard ofcomparison
D. To prevent sampling error
12. Lignins, which are responsible for evolutionary adaptation ofplants from aquatic to land possible, belong to the class
A. Alkalods
B. Proteins
C. Phenolics
D. Terpenes
13. The correct order of procedure in DNA fingerprinting is
A. DNA isolation> Restriction digestion> peR amplification> Gel electrophoresis> Southern blotting> Autoradiography> Analysis of DNA print pattern
B. DNA isolation> Restriction digestion> peR amplification> Southern blotting> Gel electrophoresis> Autoradiography> Analysis ofDNA print pattern
C. DNA isolation> PCR amplification> Gel electrophoresis> Southern blotting Restriction digestion> Autoradiography> Analysis of DNA print pattern
D. DNA isolation> PCR amplification> Restriction digestion> Gel electrophoresis> Southern blotting> Alltoradiography Analysis of DNA print pattern
14. Number of 'High Energy bonds are required to translate a 400 aa protein
A.400
B.800
C.1200
D.1600

15. A reaction medium of 1000 ml containing 10 mM Tris-HCI buffer pH 7.5, 0.5 mM MgCl2and 0.05% NaN3 has to be prepared using the stock solutions of 1 M Tris Hel pH 7.5; 100 mM MgCl2 and NaN3 solution. The volumes of stocks should be mixed as
A. 10 ml ofTris-HCI, 5 ml ofMgC12 and 50 ml ofNaN3 in 935 ml of ddH20
B. 50 ml ofTris-HCI, 5 ml of MgC12 and 25 ml ofNaN3 in 920 ml of ddH20
C. 10 ml ofTris-HCl, 2.5 ml ofMgCl2 and 50 ml ofNaN3 in 937.5 mlofddH20
D. 50 ml ofTris-HCl, 2.5 ml ofMgCl2 and 50 ml ofNaN3 in 897.5 ml of ddH20
..........,

16. Identify an agent which is NOT associated with bioterrorism or biocrimes
A. Brucella suis
B. Rickettsia prowax.ekii
C. Listeria monocytogenes
D. Yersinia pestis
17. In plant cells, nitrate reductase is located in
A. Chloroplasts
B. Mitochondria
C. Cytoplasm
D. Vacuole
18. The ABA (abscisic acid) is NOT associated with
A. Dormancy
B. Stomatal aperture
c.
Abscission

D.
Biotic stress


19. Stomatal opening is regulated by light via the action
A. Phototropin
B. Cryptochrome
C. Cryptochrome and phytochrome
D. cryptochrome and phototropin
20. Fredrick Griffith used smooth and rough strains ofStreptococcus pneumonia in his classical experiment that showed DNA might be the genetic element. Which ONE ofthe following observations gave the clue for·this discovery?
A. R strain became S strain when mixed with heat killed S strain
B. R strain remained R strain when mixed with heat killed S strain
C. S strain became R strain when mixed with heat killed R strain
D. R strain became S strain when mixed with live strain

21. A female cat with a mutant phenotype was bred with a wild-type male cat. All progeny males and 4 females) show the mutant phenotype. On the other hand, all progeny males and 4 females) from the reciprocal cross between a mutant male and wild-type female showed the wild-type phenotype. Which of the following explain the inheritance pattern of the mutation?
A. Recessive
B. Sex-linked inheritance
C. Autosomal inheritance
D. Mitochondrial inheritance
22. The genetic elements, which do not get transcribed hut control the transcription of other genes are
A. Open reading frame
B. Structural gene
c.
Promoter

D.
Coding sequence


23. An aminoacyl synthetase is responsible 'for
A. Formation of peptide bond
B. Attaching amino group to organic acid
C. Movement oftRNA from A to P sites
D. Joining aminoacid to a tRNA
24. A typical inhibitor of photosynthesis, with no effect on mitochondrial respiration
IS
A.2.4-D
B. DCMU or diuron
C.KCN

D. Methyl amine
25. A typical feature of plant mitochondria"unlike the animal mitochondria is the
occurrence of

A. Cytochrome oxidase.
B. Alternative oxidase.
C. Fo-FI complex
D.Chlorophyll


PART-B


26. Ferredoxin-dependent nitrate reduction occurs in
A. Cytoplasm
Plasma membrane

c.
Chloroplasts

D.
Xylem cells


27. The operation of photorespiratory cycle in plants requires
A. Peroxisomes only
B. Mitochondria only
C Chloroplasts only

D. All the above three organelles
28. The nlajor site of sulfate redllctioll in plallt cells is
A. Xylem vessels
B. Vacuoles
C. Mitochondria
D. Chloroplasts
29. Which ofthe following is TRUE regarding T4 terminal deoxynucleotidyl transferase?
A. does not require existing DNA strand for adding new nucloetides
B. does not require template strand
C. can add nucleotides to 5'..end of DNA strand
D. can add nucleotides to 5-end of both DNA and RNA strands
30. An important intermediate involved in biosynthesis of several products of secondary metabolism in plants
A.RuBP
B. Glucose
C. Shikimic acid
D. Phosphoglycerate
31. If non-disjunction occurs in meiosis II during gametogenesis, what will be the result at the completion of meiosis?
A. all the gametes will be diploid .
B. two gametes will be n and two will be n-1
c.
one gamete will be n one will be and two will be n

D.
two ofthe fOUf gametes will be haploid, and two will be diploid



G-b4­
32. Specialized transduction can be distinguished from generalized transduction by the fact that
A. homologous recombination is involved in the former but usually not the latter
B. transducing particles are involved in the former but usually not the latter
C. a selective. medium is required to demonstrate the former but usually not the latter
D. lysogeny is involved in the fonner but usually not the latter
33. In Drosophila melanogaster, the two genes wand sn are X-linked and 30 map units apart. A female fly of genotype sn is crossed to a male from a wild-type line. What percent ofmale progeny will be sn?
A. 35
B. 15
C. 30
D.45
34. In a self-fertilizing plant, what proportion ofa trihybrid F2 will breed true (i.e. will not segregate for any ofthe three loci)?
A. Vi
B. 1;4
C. 1/8
D. 1/64
35. Identify the mismatch with respect to scientists and their contributions from the following
A. W. H. Muir -Discovery of cytokinins
B. C. Cocking -Enzymatic isolation and culture of protoplasts
C. Morel-Propagation of orchids
D. Melchers -Production of somatic hybrids
36. What is the frequency ofheterozygotes Aa in a randomly mating population in which the frequency ofall dominant phenotypes is 0.19?
A. 0.81
·B. 0.18

C. 0.09
D.0.9
37. Ofthe two diploid species, species 1has 36 chromosomes and species II has 28 chromosomes. How many chromosomes would be found in an allotriploid individual?
A. 42or54
B. 46or50
C. 74or86
D. 84or108
38. Which of the following tool is used to predict localization of a protein with a given amino acid
A. TargetP
B.BLASTP

C. Primer-BLAST
D. Sequence Translation
39. Rubber is a
A. Monoterpene
B. Diterpene
C. Triterpene
D. Polyterpene
40. The follow requires NAD+ as a cofactor
A. T4 DNA ligase
B. Taq DNA ligase
C. T4 RNA ligase
D. T4 polynucleotide kinase
41. Reverse transcriptase
A. DNA dependent RNA polymerase
B. RNA dependent DNA polymerase
C. RNA dependent protein synthase
D. DNA dependent protein synthase
42. Nucleotide sequence of a gene from seven plant species is given to you. Which of the following tools would you use to know the homology between the sequences?
A. Hydropathy analysis
B. ChloroP
C. ClustalW
D. Primer Express

43. The appropriate method to separate the isoforms ofproteins
A. UV/visible spectroscopy
B. 2-dimensional electrophoresis
C. SDS-PAGE
D. Agarose gel electrophoresis
44. Secondary structure of proteins can be determined by
A. Fluorescence spectroscopy
B. UV/visible spectroscopy
C. Circular Dichroism
D. Flame photometry
45. 3-Dimensional structure ofproteins will be solved through:
A. Fourier transform infrared spectroscopy
B. X-Ray crystallography
C. Electron microscopy
D. Auto radiography
46. Location of the protein cleavage by trypsin
A. Lysine and Arginine
B. Lysine and Tyrosine
C. Arginine and glutamine
D. Phenylalanine and Lysine
47. Potato is the modification of
A. Root
B. Stem
C. Bark
D. Leaf
48. Fructose-bisphosphate splits into Triose phosphates by the action ofthe enzyme
A. fructae
B. phosphatase
C. aldolase
D. esterase 49. tRNA is involved in the biosynthesis of

A. starch
B. nucleic acids
C. vitamins
D. proteins
50. Protein phosphorylation is
A. Glycosylation
B. Oxidation
c.
Posttranslational modification

D.
Methylation


51. During what stage ofphotosynthesis are ATP and NADPH converted to ADP Pi andNADP+?
A. The light-independent reactions
B. The light-dependent reactions
C. AandB
D. Cyclic photophosphorylation
52. Find out the mismatch in the infrared spectroscopy
A. -eH3bending -1460, 1365
B. -e-c-stretching -1165
C. stretc.hing -2960
D. stretching -2720
53. An association coefficient that ignores any characters that both lack is
A. Simple matching coefficient
B. Jaccard coefficient
C. Both SSM and 8J
D. Similarity matrix
54. An interim taxonomic status for uncultured organism is refereed as
A. "Candidatus"
B. "Candus"
C. "Interimus "
D. "Nomus "


55. Animals/plants which are microorganism free are called
A.Gnotobiotic
B. Xenobiotic
c.
Gentobiotic

D.
Genobiotic


56. Which among the following is a negative interaction
A. Protocooperation
B. Commensalistic
C. Amensalism
D. Mutualism
57. An example of endomycorrhizae is
A. Gigaspora
B. Cennococcum
c.
Pisolithus

D.
Amanita


58. Amongst the following photoreceptors, which is the most recently discovered?
A. Aureochrome
B. Neochrome
C. Cryptochrome
D. UVR8
59. If the new leaves are pale green, turn yellow-green as they enlarge; plants show
sparse growth; lack fruits or with few fruits, pale in color, the plants are said to be deficient in
A. Nitrogen
B. Copper
C. Boron
D. Magnesiwn
60. Which of the following is the cellular location for ethylene receptors?
A. Plasma membrane
B. Endoplastic reticulum
C. Cytoplasm
D. Nucleus

61. Graminaceous plants acquire iron with the help of
A. Phytoalexins
B. Phytosiderophores
C. Metallothionin
D. Thioredoxin
62. Which ofthe following is the odd one?
A. TILLING
B. RNAi
c.
Virus-induced gene silencing

D.
AFLP


63. The genetic recovery and investigation of all the products from a single meiotic event in Neurospora, the bread mold causing fungus is possible by
A. Tetrad analysis
B. Heterokaryon analysis
c.
Homokaryon analysis

D.
Monad analysis


64. Given the antisense strand DNA codon TAC the anticodon that pairs corresponding mRNA codon could be
A.
B.
C.
D.
65. A diallel mating refers to
A. Mating between two individuals only
B. Mating between two different alleles only
C. Binary mating
D. Mating in all possible combinations
66. Which among the following has a transcribed structural gene product but do not get translated into protein .
A. tRNA and rRNA
B. mRNA and tRNA
C. rRNA and mRNA
D. mRNA alone


67. Pseudodominance may be observed in heterozygotes for
A. A deletion
B. A duplication'
C. A reciprocal translocation
D. A paracentric inversion
68. The expected ratio for dominant epistasis is
A.9:3:4

B.


C.


D.
12:3:1


69. What do the biologists call a group ofiridividuals ofthe same kind occupying a given area at the same time?
A. The biosphere
B. A population
C. An ecosystem
D. A community
70. To which kingdom do all the eukaryotic, single-celled organisms belong?
A. Archaebacteria
B. Plants
C. Eubacteria
D. Protistans
71. Stevioside is
A. an alkaloid
B. a sweet protein
C. a non-saccharide sweetner
D. a powerful anaesthetic agent
72. How does carbon-14 differ from carbon-12?
A. 14C llas more neutrons
B. 14C has luore electrons
c.
14C has nlore protons

D.
14C is all ion



73. What type ofbonds hold together tIle two strands ofa DNA molecule?
A. Hydrogen bonds
B. Single covalent bonds
c.
Double covalent bonds

D.
Ionic bon,ds


74. What is population 'variable as defined as?
A. Populatiol1 size
B. Populatio density
C. Net reproduction per individual per unit time
D. Net death rate per individllal per tmit time
75. When unifonn population dispersion does occur in 11ature, it tends to be result of which ofthe following?
A. Competition
B. Sytnbiosis
c.
Social bellavior

D.
Interference