Master of Science II (Analytical Chemistry)
Examination: Oct Nov 2016 Semester III (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SD
262
Wednesday
16/11/2016
2:30 P.M
to
5:00 P.M
Advance Separation
Techniques
C
IX
Instructions: Attempt in all five questions.
Section-I is compulsory.
Attempt any two questions from Section II and any two
questions from Section III.
Answers to all questions should be written in one answer book.
All questions carry equal marks.
Figures to the right indicate full marks.
Use of log table and non-programmable calculators in allowed.
Total Marks:70
SECTION I
Q.1 Attempt the following questions: 14
Give two important features of affinity chromatography.
Explain role of stationary phase in paper chromatography.
Define the term Rf-value.
Mention the names of chromate graphic methods in which liquid is the mobile
phase.
Give two examples of extractants.
Explain in brief electro-osmotic flow.
How is the interaction between ligand and target protein molecule is reversed.
Give two applications of ultrafiltration.
Explain the term current efficiency in electrophoresis.
What is zone electrophoresis.
Diethyl ether is better extractant than dichloro-diethylether. Explain.
Why spacer-arms are used in gel permeation chromatography.
Why chaotropic agents are avoided for elusion.
Which compound is dissolved in dialysate solution of peritonial dialysis? and
Why?
SECTION II
Q.2 Describe methods employed for locating spots in paper chromatography. How
the Rf-values and Rx-values are calculates?
07
Which gels are most commonly used and why? Give two examples of rigid
gels with their molecular weight exclusion limits?
07
Q.3 Explain Haemodialysis with the help of diagram. 07
Explain the principle, experimental procedure and applications of capillary
electrophoresis.
07
Q.4 Explain four steps involved in purification of mixture by affinity
chromatography.
07
Explain principle and process of zone-refining technique. 07
SECTION III
Q.5 Differentiate between paper and column chromatography. 05
Explain the term micellar electro kinetic capillary chromatography. 05
Write note on solid-phase extraction. 04
Q.6 What is current efficiency in electro dialysis? Explain with the help of
equation.
05
Give principle and classification of extractors. 05
Explain with example the Zone-electrophoresis. 04
Q.7 Write short notes on any THREE 14
Radial chromatography
Components involved in affinity chromatography.
Applications of dialysis.
Factors affecting solvent extraction