Master of Science II (Analytical Chemistry)
Examination: Oct Nov 2016 Semester III (Old CGPA)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SD
257
Wednesday
16/11/2016
02:30 P.M
to
05:00 P.M
Advance Separation
Techniques
C
IX
Instructions: Attempt in all five questions.
Section I is compulsory.
Attempt any two questions from Section II and any two
questions from Section III.
All questions carry equal marks.
Figures to right indicate full marks.
Use of log table and non-programmable calculator in allowed.
Total Marks:70
SECTION I
Q.1 Attempt the following questions. 14
Explain the term permeate in ultrafiltration.
Define the term Rf-value.
Define the term spacer arms.
What is the meaning of MMD.
Define the term distribution coefficient.
Give two important features of solvents extraction.
Give long form of the term MECC.
What is the synergic extraction?
Explain stationary phase in chromatography.
Mention the types of the membranes used as ultrafiltration membranes.
Define distribution ratio.
Define exclusion limit.
Diethyl ether is better extractant than -dichlorodiethyl ether. Explain.
Define the term electrophoresis.
SECTION II
Q.2 What is the principle of paper chromatography? How are the components of a
mixture identified by paper chromatography?
07
What are the applications of gel permeation chromatography? Explain the role
of ligand in gel permeation chromatography.
07
Q.3 Describe the steps involved in affinity chromatography. 07
Explain in brief the technique of solvent extraction. 07
Q.4 Write a note on hemodialysis. 07
Explain the working technique of electro dialysis with the help of diagram. 07
SECTION III
Q.5 Explain the technique of ultrafiltration. 05
Give applications of dialysis. 05
Explain in brief the extraction by chelation. 04
Q.6 Give applications of solvent extraction. 05
Explain the role of rigid gels in gel permeation chromatography. 05
What is isoelectric focusing? Explain with suitable examples. 04
Q.7 Write a note on any three of the following: 14
Ascending chromatography
Applications of gel permeation chromatography
Steps involved in affinity chromatography
Factors affecting solvent extraction