M.Sc. (Semester III) (CBCS) Examination Mar/Apr-2018
Analytical Chemistry
ADVANCED SEPARATION TECHNIQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: Attempt in all five questions. Question one is compulsory. Attempt any two questions from question number 2-4 and any two questions from question numbers 5-7. Answer to all questions should be written in same answer book. All question carry equal marks. Figures to the right indicate full marks. Neat labeled diagrams should be drawn wherever necessary. Use of log table and nonprogrammable calculator is allowed.
Section I
Q.1

Attempt the following:
10
Give steps involved in chromatographic methods.
Define the term Rx value.
Mention types of interactions between ligand and target protein molecule.
Explain in short current efficiency in electrodialysis.
Define the term critical pressure.
Define the term Mie scattering.
Give application of DLS.
Give two examples of semi-rigid gels.
What is distribution coefficient?
10) Define synergic extraction.

Fill in the blanks:
04
paper is used as stationary phase in paper chromatography.
The unit of migration velocity in electrophoresis is
With increase in pressure the density of supercritical fluid
Electrostatic process is used for controlling
Section II
Q.2
Explain principle and working of dialysis with the help of diagram.
07
What is the principle of affinity chromatography? Describe components involved in affinity medium.
07
Q.3
Which gels are commonly used in gel permeation chromatography? Explain role of ligand and spacer armsin gel permeation chromatography.
07
Explain sedimentation constant and sedimentation equilibrium of ultrafiltration.
07
Q.4
Explain factors affecting solvent extraction.
07
What is the principle in zone refining technique? Explain process of Zone refining.
07
Section III
Q.5
Describe ascending paper chromatography.
05
Explain the elusion methods used in affinity chromatography.
05
Explain in brief the technique of solvent extraction.
04
Q.6
Define and explain the term Miceller electro kinetic capillary chromatography.
05
Give applications of dialysis.
05
Explain the technique of ultrafiltration.
04
Q.7
Write a note on any Three of the following.
14
Solid phase extraction
Two dimensional chromatography
Peritoneal dialysis
Zone electrophoresis