M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Analytical Chemistry
ADVANCE SEPARATION TECHNIQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: All questions are compulsory. Draw neat and labeled diagram and give equations wherever necessary. Figures to the right indicate full marks.
Q.1
Select the most correct alternative from the following and rewrite the sentences.
14
Gel filtration is technique that fractionates substances largely according to their size.
Ionic
Molecular
Atomic
All of these
Ion exchange resin is used as the phase.
immobile
mobile
transfer
equilibrium
The movements of substances relatives to the solvent is expressed in terms of value.
Rf
Rx
Ra
Rs
The paper spotted with the samples is first developed using one solvent system as the phase.
transfer
mobile
immobile
equilibrium
In solvent extraction two phases are namely aqueous and phase.
Inorganic
Physical
Organic
Equilibrium
The extraction of uranium with 8 hydroxyquiline in
Acetone
Chloroform
Alcohol
Benzene
The stability of metal complexes with ionic potential.
increases
decreases
lower
none of these

The basic methods used in liquid-liquid extraction.
two
one
three
all of these
The proper conditions for the separation can be predicted by theory.
Mulliken
Hukel
Plate
All of these
10) The solubility of the salts in the oxygen containing solvents depends upon specific interaction of the solvent oxygen and the
solute
solubility
solvent
effluent
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SLR-VF-181
11) The term electro chromatography is used with reference to the physical transport of charged
solvent
solutes
ions
particles
12) There are various factors which affects migration rates in electrophoresis.
capillary
curtain
spot
zone
13) The electro chromatographic homogeneity of zones is an important criteria in the establishment of
ions
particles
atoms
purity
14) Fresh water tends to move through a membrane.
dark
transparent
polymembrane
semipermiable
Q.2
a)Answer the following (any Four)
08
Give steps involved in chromatographic methods.
Mention the detector used in ion chromatography.
Explain in short current efficiency in electrodialysis.
Give two examples of semi-rigid gels.
Define synergic extraction.
b)Write notes on (Any Two)
06
Zone electrophoresis
Peritoneal dialysis
Two dimensional chromatography
Q.3
a)Answer the following (Any Two)
08
Explain technique of ultrafiltration
Explain the term Miceller electro kinetic capillary
Explain in brief the techniques of solvent extraction.
b)Answer he following (Any One)
06
What is the principle in zone refining technique? Explain process of Zone refining.
What is principle of capillary electrophoresis? Explain in brief its sample preparation.
Q.4
a)Answer the following (Any Two)
10
Describe ascending paper chromatography.
Discuss the electro dialysis cells.
Explain in brief the technique of solvent extraction.
b)Answer the following (Any One)
04
Explain the elusion methods used in affinity chromatography.
Discuss the extraction equilibria for solvation.
Q.5
Answer the following (Any Two)
14
a)What is the principle of affinity chromatography? Describe components involved in affinity medium.
b)Explain factors affecting solvent extraction.
c)Give applications of electrophoresis in inorganic and organic separations.