Master of Science II (Biotechnology) Examination: Oct Nov 2016
Semester III (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SK
115
Friday
18/11/2016
02:30 P.M
to
05:00 P.M
Genetic Engineering
C
X
Es Instructions: Section-I is compulsory.
From Section II attempt any four.
All questions carry equal marks.
Figures to right indicate full marks.
Draw neat and labeled diagrams.
Total Marks: 70
Section-I
Q.1 Multiple Choice questions:
CaMV is an
DNA containing Virus Animal Virus
RNA containing Virus Bacteriophage
The first human protein produced through recombinant DNA technology is
Insulin Erythropoitin
Interferon Somatostatin
Bt cotton is not:
A GM plant Insect resistant
A bacterial gene expressing system Resistant to all pesticides
A obtained by a sexual reproduction is an
Group of genetically similar
organisms
Population
Assembly of genes and protein None
The trigger for activation of toxin of Bacillus thuringiensis is
Acidic pH of stomach High temperature
Alkaline pH of gut None of the above
A Plant called Rauwolfia serpentina is under the threat of extinction. To
save this plant, which technique is highly useful?
DNA finger printing Hybridoma technology
Genetic engineering In vitro culture
Endonucleases, a group of enzymes cleave DNA
Externally Internally
Both a and b Neither a nor b
Page 1 of 2
Define the following terms:
YAC and BAC
Endonucleases
Primers
cDNA
Marker genes
Bio-spharming
Plasmid
Section-II
Attempt any four
Q.2 Long answer type
Add a brief note on DNA sequencing by Maxam and Gilbert method
Q.3 Long answer type
With suitable example and application explain vector mediated gene transfer
Q.4 Long answer type
Add a brief note on expression system yeast and bacteria with suitable example?
Q.5 Long answer type any Two:
Explain in detail the construction of Genomic library
Add a note on DNA fingerprinting
Explain in detail DNA Amplification by PCR
Q.6 Write shorts note on any TWO of the following:
Genetically engineered animals
Assembly of genes
Cosmid Vector