M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Biotechnology
GENETIC ENGINEERING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Choose the correct option and rewrite the sentence:- 14
classes of restriction enzymes are there?
3 1
2 4
pBR322 has of the following selection marker.
Kanr Tetr
Actr Str r
The virus mediated gene transfer using genetically modified bacteriophages is
called
Transfection Transformation
Transduction Conjugation
different types of chemical treatments are required in Maxam-
Gilbert method.
2 3
1 4
is the mode of action of exonuclease III.
Exonuclease III acts on double stranded DNA in direction
Exonuclease III acts on double stranded DNA in direction
Exonuclease III acts on single stranded DNA in direction
Exonuclease III acts on single stranded DNA in direction
Vectors designed to replicate in cells of two different species are called

Plasmid vector Shuttle vector
Phasmid vector Phagemid vector
The removal of tumor causing genes from Ti plasmid is termed as
Gene replacement Insertional inactivation
Disarming Gene cloning
Reverse transcriptase PCR uses
tRNA as template rRNA as template
RNA as template mRNA as template
The variation in number of tandem repeats between two or more individuals is
called
VNTR RFLP
PCR Chromosome walking
10) The ability of cells to take up DNA fragments from surrounding is called

Transduction Transformation
Transfection Conjugation
Page 2 of 2
SLR-VE-80
11) will be the transcription product of 3'-AUCCGAGCUAAC-5' when
treated with reverse transcriptase.
3'….GTTAGCTCGGAT….5' 3'….AUCCGAGGAUUG….5'
5'….GTTAGCTCGGAT….3' 5'….UAGGCUCGAUUG….3'
12) vector can be propagate cloned fragments up to
20-25 kb 6-7 kb
10-20 kb 15-20 kb
13) All the following are thermostable polymerases except
Taq polymerase Pfu polymerase
DNA polymerase III Vent polymerase
14) The term 'endonuclease' refers to cutting the DNA sequence from
Exactly in the middle of the chain
The ends of the chain
Anywhere in the chain
Only within the polynucleotide chain, not at the ends.
Q.2 Answer the following any four:-
1. Draw the structure of M13 vector.
2. Define ligase with examples.
3. Write a note on cosmids.
4. Define transgenic animals.
5. Draw the structure of TMV.
08
Write note on any two
1. Write notes on Restriction endonuclease.
2. Write notes on Baculovirus.
3. Write notes on c-DNA library.
06
Q.3 Answer the following (Any
1. Explain electroporation method.
2. Describe preparation primers and probes.
3. Write a note on RFLP.
08
Answer the following any
1. Give a detailed account on bacteriophage.
2. Explain in detail genetically engineered biotherapeutics.
06
Q.4 Answer the following. (Any Two)
1. Write in detail Maxam and Gilbert method.
2. Explain direct recombinant screening method.
3. Write a note on CaCl microinjection method of transformation.
10
Answer the following any
1. Write a note on southern blotting.
2. Give a detailed account on phagemids.
04
Q.5 Answer the following. (Any Two) 14
Describe in detail gene therapy.
Explain in detail isolation and purification of vector DNA
Write a note on Microarray.