M.Sc. (Semester III) (CBCS) Examination Mar/Apr-2018
Biotechnology
GENETIC ENGINEERING
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I is compulsory. Answer any Four questions from section II.
Section I
Q.1

Rewrite the sentence after using correct alternative given below:-
07
First step of genetic engineering is
Isolation of gene interest
Insertion of gene into vector
Growth of GMO
Expression of gene
Which of the following cannot be used as a vector?
Phage
Plasmid
Bacterium
All can be used as vector
The enzyme used in the polymerase chain reaction is
Restriction endonuclease
Reverse transcriptase
DNA polymerase
RNA polymerase
In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to
Isolate fragments of cloned bacterial plasmids
Isolate fragments of frog DNA that contained an rRNA gene.
Cleave the bacterial plasmid
All of these are correct
A probe is used in which stage of genetic engineering?
Cleaving DNA
Recombining DNA
Cloning
Screening
Which of the following is not an application of genetic engineering in plants?
Nitrogen fixation
DNA vaccines
Resistance to glyphosate
Production to insecticidal proteins in plants
Bacterium which is able to synthesize human growth hormone is known as
E.coli bacterium
H.coli bacterium
X.coli bacterium
I.coli bacterium

Define the following terms.
07
Gene
Vectors
Probes
RFLP
Gene therapy
Phagmid
PCR
Page 2 of 2
SLR-UE-94
Section II
Q.2
Explain in details vectors properties and structure of natural and artificial plasmids.
14
Q.3
Discuss about the construction of genomic DNA and cDNA library.
14
Q.4
Describe the Blotting techniques.
14
Q.5
Answer any two from the following:
14
Colony hybridization
Sanger's method of sequencing
DNA fingerprinting
Q.6
Write short notes on. (Any two)
14
DNA manipulation enzyme
Mammalian Vector
Transformation