M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Biotechnology
GENETIC ENGINEERING
Day Date: Saturday, 18-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: All questions of Section I are compulsory.
Answer any Four questions from section II.
All question carry equal marks.
Section I
Q.1 Rewrite the sentence after using correct alternative given below:- 07
Restriction enzymes are
DNA unwinding enzymes DNA joining enzymes
DNA cleaving enzymes None of these
2 Ti plasmid used in genetic engineering is obtained from
Bacillus thringiensis Agrobacterium rhizogenes
Agrobacterium tumefaciens Bacillus subtilis
3 Who discovered recombinant DNA (rDNA) technology?
Har Gobind Khorana J. D. Watson
Sutton and Boveri Stanely Cohen and Herbert Boyer
4 Which of the following is used as vector in gene theraphy for SCID
Arbovirus Rotavirus
Parvovirus Retrovirus
5 Get Electrophoresis separate DNA fragments according to their
Percentage of labelled nucleotides Base sequence
Electrical charge Size
6 Why is it more difficult to create transgenic animals than transgenic
plants?
Plants and animals use a different genetic code
Animals cells cannot replicate foreign DNA
It is more difficult to introduce foreign DNA into animal cells.
Animal cells cannot transcribe and translate foreign DNA
7 Which enzymes are used to cut large segments of DNA into
fragments for DNA fingerprinting?
Reverse transcriptase Restriction enzymes
DNA polymerase DNA ligase
Define the following terms. 07
Restriction enzyme
Expression Vector
cDNA library
DNA chips
Electroporation
Biopharming
Plasmid.
Page 2 of 2
SLR-ME-107
Section II
Q.2 Explain in details of the expression vectors used in the cloning? 14
Q.3 Discuss the methods of screening in rDNA technology. 14
Q.4 Describe in details DNA sequencing methods. 14
Q.5 Answer any two from the following: 14
Transgenic animals
Gene gun
Genomic Library
Q.6 Write short notes on. (Any two) 14
Shuttle vector
Bacteriophages vector
RAPD.