Master of Science I(Microbiology) Examination: October 2016
Semester III (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SO
630
Wednesday
16/11/2016
02.30 PM
To
05.00 PM
Molecular Biology and
Genetic Engineering
C
IX
Instructions: Part I is compulsory.
Attempt any four questions from Part II.
Part I and Part II should be written in same answer book.
Figures to the right indicate full marks.
Total Marks:70
PART I
Q.1 Rewrite the following sentences by selecting correct answers from given
alternative.
14
DNA finger printing technique was developed by
Jeffreys, Wilson and Thien Boysen and Jensen
Edward Steptoe
Recombinant DNA technology is also called
biotechnology modern biotechnology
genetic engineering transgenic technology
RT-PCR
Reverse transcriptase polymerase chain reaction
Rightward tmp polymerase chain reaction
Rotating tube polymerase chain reaction
None of these
Genetic modification brought about by a virus in bacteria is known as
Transformation Transduction
Conjugation Transfection
T-4 polynucleotide kinase is used for
Labeling ends of DNA Labeling ends of DNA
Creating blunt ends of DNA Dephosphorylation of DNA
PCR amplification cycle involves
A. Denaturation
B. Primer annealing
C. DNA polymerization
D. Reaction mixture containing g target DNA, primer, therostable DNA
polymerase and dNTP
C and D B and C
C and D C and D
Shotgun approach is used for construction of
cDNA library Genomic library
Both a and b None
Expression vectors contain a sequence known as
A ribosome binding site An antibiotic resistance marker
A multiple cloning site An orisitr
The most widely used vectors for DNA library construction is

Plasmid (lambda) phage
YAC PAC
10) Nonsense codons are present on
m RNA t RNA
r RNA DNA
11) MaxamGinibert sequencing requires radioactive labeling at
7"

12) Protein-protein hybridization results in
Southern blotting Northern blotting
Western blotting None of these
13)BAC is derived from
Cole plasmid F plasmid
plasmid M13 plasmid
14) not a component of YAC.
Centromere Telomere
Origin of replication Cos site
PART II
Q.2 Write in detail on Vectors used in genetic engineering. 14
Q.3 Discuss in detail the Construction of recombinant DNA molecule. 14
Q.4 Write short answer on any two of the following: 14
Describe in detail role of restriction endonucleases in r-DNA technology.
Describe in detail molecular biology of nitrogen fixation.
What is mutation? Describe in detail fluctuation test.
Q.5 Write short answers any two: 14
Explain in detail DNA sequencing and add a note on PCR sequencing.
Briefly describe molecular biology of oncogenesis.
Describe in detail metabolic engineering.
Q.6 Write short notes on any two: 14
Briefly describe applications of genetic engineering.
Briefly describe DNA libraries.
Describe in detail Nucleic acid hybridization