M.Sc. (Semester III) (CBCS) Examination Mar/Apr-2018
Microbiology
MOLECULAR BIOLOGY AND GENETIC ENGINEERING
Time: 2½ Hours
Max. Marks: 70
Instructions: Part- Questions No.1 Compulsory. Attempt any 4 questions from Part II. Figures to the right indicate full marks. Answer to the Part I and Part II are to be written in same answer booklet only.
Part I
Q.1
Rewrite the sentence by choosing correct alternative from the following:-
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Amplification of plasmids is carried out by
Penicillin
Streptomycin
Chloramphenicol
Tetracycline
A cloning vector consisting of CoS site inserted in a plasmid used to clone DNA fragment of lambda phage is
phage mid
cosmid
plasmid
YAC
is Tumor inducing plasmid.
pBR322
Ti
PUC19
PUC18
Bioremediation is
Use of genetically engineered organism in pharmaceutical industry
Biological control
Biopurification of environment
Both b c
is produced through rDNA technology.
Insulin
Penicillin
Polio vaccine
Azydothymidine
Southern blotting technique used to separate
DNA
RNA
protein
amino acids
The role of plasmid in conjugation was first described in
Pseudomonas
E. coli
Rhizobium
pneumonococcus
Genetic engineering involve
conjugation
cloning
mutations
deamination
Genetic modification brought about by a virus in bacteria is known as
transformation
transduction
conjugation
transfection
Page 2 of 2
SLR-UQ-539
10) is called the „Natural Genetic Engineer‟.
Azotobacter
Pseudomonas
Agrobacterium
Rhizobium
11) enzyme is called as Molecular scissor.
Restriction endonuclease
helicase
ligase
exonuclease
12) The PCR is used to
amplify a small amount of DNA
cleave bacterial plasmids
seal sticky ends
identify target plasmids
13) Genetic modification brought about by a cell to cell contact in bacteria is known as
transformation
transduction
conjugation
transfection
14) Restriction endonucleases are obtained from
bacteria
all prokaryotic cell
bateriophages
plasmid
Part II
Q.2
What is recombination? Describe in detail Gene transfer in bacteria with reference to transformation, transduction and conjugation.
14
Q.3
What are Vectors? Discuss in detail how they are significant in Genetic engineering?
14
Q.4
Write short answer on any two of the following:
Briefly describe theories of Oncogenesis.
Discuss in detail chemical and biological mutagenic agents.
Explain in detail screening of recombinants.
14
Q.5
Write in short on any Two of the following:
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Briefly describe methodology and applications of Protein Engineering.
Comment on "synthesis of low molecular weight compounds by metabolic engineering"
Justify the statement "nucleic acid hybridization is best tool in bacterial taxonomy.
Q.6
Write short notes on any Two of the following:
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Restriction endonuleases
Applications of Genetic engineering
Insertion of recombinant DNA