M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Microbiology
MOLECULAR BIOLOGY AND GENETIC ENGINEERING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagram wherever necessary.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
Maxam Gilbert sequencing requires radioactive labeling at end
of DNA.

Neither nor
labelling method works with both short DNA and long DNA.
Nick translation Radioactive labeling
Non-radioactive labeling None of these
can be used to build genomic libraries.
Chromosome Cosmids
Phagemids Nucleosomes
A cloning vector consisting of CoS site inserted in a plasmid used to clone
DNA fragment of lambda phage is
Phage mid Plasmid
YAC Cosmids
PCR amplification cycle involves
A. Denaturation
B. Primer annealing
C. Reaction mixture containing target DNA, primer, thermostable DNA
polymerase and dNTP
D. DNA polymerization
C and D B and C
C and D A,C and D
The concept of Genetic load applied to humans in 1950 by
JBS Haldane H.J. Muller
Allan Maxam Gilbert
enzyme is known as Molecular Scissor.
Exonuclease Helicase
Restriction endonuclease Ligase
A probe is used in stage of genetic engineering.
Cleaving DNA Screening
Cloning Recombining DNA
is not a component of YAC.
Cos site Telomere
Origin of replication Centromere
Page 2 of 2
SLR-VP-436
10) RT-PCR means
Reverse transcriptase polymerase chain reaction
Rightward template polymerase chain reaction
Rotating tube polymerase chain reaction
Revolving tube polymerase chain reaction
11) is called the „Natural Genetic Engineer‟.
Azotobacter Rhizobium
Pseudomonas Agrobacterium
12) Recombinant DNA technology is also called as
Biotechnology Nano biotechnology
Genetic engineering Transgenic technology
13) Genetic modification brought about by a cell to cell contact in bacteria is
known as
transformation transduction
conjugation transfection
14) is a Tumor inducing plasmid.
pBR322 Ti
PUC19 PUC18
Q.2 Define and explain any four of the following:-
1. Hybridization Probe
2. C-Value Paradox
3. DNA Libraries
4. Shuttle vectors
5. Nucleic acid Hybridization
08
Write notes on any two of the following:-
1. Genome libraries
2. Protoplast fusion
3. Fluctuation test
06
Q.3 Answer Any Two of the following
1. Explain in detail Law of DNA constancy and redundancy.
2. Briefly describe Insertion of recombinant DNA.
3. Explain in detail role of restriction endonucleases in Genetic Engineering.
08
Answer any one of the following
1. Briefly describe metabolic engineering and add a note on its significance.
2. Comment on "Molecular biology of Nitrogen fixation".
06
Q.4 Answer Any Two of the following
1. Briefly describe Protein engineering.
2. Describe briefly theories of Oncogenesis.
3. What are Vectors? How they are significant in Genetic engineering?
10
Answer any one of the following
1. What is recombination? Briefly describe transformation process.
2. Briefly describe role of plasmids in recombinant DNA technology.
04
Q.5 Answer any two of the following 14
What is DNA sequencing? Discuss in detail different methods of DNA
sequencing.
Justify the statement "nucleic acid hybridization is best tool in bacterial
taxonomy".
Comment on "Natural bacterial recombination process helps for mutation in
bacteria".