M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Microbiology
MOLECULAR BIOLOGY AND GENETIC ENGINERRING
Day Date: Thursday, 16-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Part- Questions No.1 Compulsory.
Attempt any 4 questions from Part II.
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same answer booklet
only.
Part I
Q.1 Choose the correct alternative given in the bracket. 14
a Tumor inducing plasmid.
pBR322 Ti
PUC19 PUC18
Southern blotting technique used to
DNA RNA
Protein Lipid
The presence of a plasmid in a bacterial culture is usually determined by

Blue white screening
Growth in the presence of antibiotic
A restriction enzyme digest
Agarose gel electrophoresis
Genetic modification brought about by a virus in bacteria is known
Transformation Transduction
Conjugation Transfection
Expression vectors contain a sequence known
A ribosome binding site An ori sit
A multiple cloning site An antibiotic resistance marker
PCR amplification cycle
Denaturation
ii) Primer annealing
iii) DNA polymerization
iv) Reaction mixture containg target DNA, primer, thermostable DNA
polymerase and dNTP
A C and D A B and C
B C and D A B C and D
not a component of YAC.
Centromere Telomere
Origin of replication Cos site
are sometimes called chimeric DNA.
cDNA rDNA
dDNA nDNA
Page 2 of 2
SLR-MQ-592
RT-PCR
Reverse transcriptase polymerase chain reaction
Rotating tube polymerase chain reaction
Rightward template polymerase chain reaction
Revolving tube polymerase chain reaction
10) commercially produced by rDNA technology.
Insulin Penicillin
Polio vaccine Azydothymidine
11) A probe is used stage of genetic engineering.
Cleaving DNA Recombining DNA
Cloning Screening
12) A cloning vector consisting of CoS site inserted in a plasmid used to clone
DNA of phage
Phage mid Cosmids
Plasmid YAC
13) In needles are used to inject DNA into cells.
Electrophoresis Microinjection
Electroporation Phage bombardment
14) Recombinant DNA technology is also called as
Biotechnology Nano biotechnology
Genetic engineering Transgenic technology
PART II
Q.2 Describe in detail the general Strategy of gene cloning. 14
Q.3 Write short essay on Protein engineering and add a note on metabolic
engineering:
14
Q.4 Write short answer on any two of the following:
What is Mutation? Discuss in detail chemical and biological mutagenic
agents.
Applications of Genetic engineering and its legal aspects.
Genome libraries and cDNA libraries.
14
Q.5 Write in short on any Two of the following: 14
Briefly describe nucleic acid hybridization and its applications in bacteria
taxonomy.
Comment on "Molecular biology of Nitrogen fixation"
Justify the statement "Natural bacterial recombination process helps for
mutation in bacteria".
Q.6 Write short notes on any TWO of the following: 14
Law of DNA constancy and redundancy
DNA sequencing
Neoplastic transformation