Master of Science I (Microbiology) Examination: Oct Nov 2016
Semester II (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SO
618
Thursday
17/11/2016
10.30 AM
to
01.00 PM
Microbial Genetics
C
V
Instructions

Part Q.1 is compulsory.
Attempt any four questions from Part II.
Part I and Part II should be written in same answer book.
Figures to the right indicate full marks.
PART I
Q.1 Rewrite the following sentences by selecting correct answers from given
alternative.
14
The exact point where nucleotides are being added to growing daughter
strands during DNA replication are called as
the origin the double helix
the template the replication fork
During transcription newly synthesized RNA forms base pairing with coding
sequence
for short distance for whole length
for no length half length
Translation and transcription is generally coupled in
Bacteria Viruses
Plants Animals
Model for replicative transposition was proposed by
McClintock Shapiro
Luria Dupra
Sometimes you can see mRNA molecules growing in both directions of
dsDNA of bacterial chromosome; it is due to fact that
RNA polymerase can work in both directions
Opposite strands of a DNA sequence code always for two different genes
genes can be transcribed from opposite strands
None
Biomolecules first arose by
genetic evolution chemical evolution
biological evolution randomly
In PCR DNA polymerase is used.
Type II Type IV
Type III Type I
Page 2 of 2
Jumping genes were discover by
Barbara McClintock Beadle and Tatum
Lederberg Delbruck
The mathionine carried by Archaeal initiator tRNA is
N-formylated non-Nformylated
N-methylated a c
10) Proofreading and mismatch repair in DNA is carried out by
DNA polymerase I DNA polymerase II
DNA polymerase II Exonuclease
11) has the smallest chromosome?
Mycobacterium E. coli
Salmonella Mycoplasma
12) Coordinated regulation of a function in bacteria is brought about by regulation
of synthesis of polycistronic mRNA regulated through
a single signal signals coordinated by Rho
double signal sigma factor
13) In the Lac-operon the genes in the operon are
always expressed only expressed when lactose is present
never express only expressed when lactose is absent
14) Overlapping genes are found in
∅X 174 E. coli
bacteriophage MS
PART II
Q.2 Describe in detail the Post transcriptional processing in Prokaryotes. 14
Q.3 Describe in detail detection, purification, amplification and rearrangement of
plasmids.
14
Q.4 Discuss in detail structural organization of bacterial chromosome and add a note
on different models.
14
Q.5 Write short answers any two: 14
Discuss briefly the "Cis Trans test".
Give brief account of DNA Finger printing.
Describe Operon model with reference to tryptophan operon.
Q.6 Write short answers any two: 14
Alternative forms of DNA
Structure of rRNA and tRNA
Describe in detail Terratogenesis