M.Sc. (Semester II) (CBCS) Examination Oct/Nov-2017
Microbiology
MICROBIAL GENETICS
Day Date: Friday, 17-11-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Part- Questions 1 is Compulsory.
Attempt any 4 questions from Part II.
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same answer booklet
only.
PART I
Q.1 Choose the correct alternative given in the bracket. 14
Sometimes you can see mRNA molecules growing in both directions of
dsDNA of bacteria chromosome; it is due to fact
RNA polymerase can work in both directions.
Opposite strands of a DNA sequence always code for two different
genes.
Genes can be transcribed from opposite strands.
None
Bacteria can decode their mRNA with as few
32 t RNAs. 30 t RNAs.
61, tRNAs. 20 t RNAs.
The replicon encoding genes essential for the cell survival is called as
Genome Chromosome
Codon Proteome
Synthesis of RNA primers for DNA chain elongation is carried out
RNA polymerase RNA primase
DNA polymerase RNA transcriptase
Model for replicative transposition was proposed
McClintock Shapiro
Luria Dupra
SOS response brings to halt
DNA RNA
Protein Carbohydrate
pBR 322 is
an original plasmid modified plasmid
viral genome a transpoons
The methionine carried by Archaeal initiator tRNA is
N-formylated, non-Nformylated
N-methylated a b
Page 2 of 2
SLR-MQ-584
Ti plasmid are found in
E-coli Streptococcus pneumoniae
Agrobacterium tumifacians Pseudomonas spp.
10) DNA replication by semiconservative mode in E.coli was experimentally
proved
Watson and Crick Meselson and Stahl
Zinder and Lederberg Delbruck Delbruck
11) E-coli polunucletide ligase requires its activity.
FAD FMN
NAD NADP
12) The coding sequences on the eukaryotic genes are termed
introns exons
split gens interrupted genes
13) In Archaeae translation is matching more
Bacteria Yeasts
Eukaryotes Mitochondria
14) Helix unwinding during replication is accomplished
DNA helicases DNA gyrase
DNA polymerase I DNA polymerase II
PART II
Q.2 Write an essay on translation in prokaryotes. 14
Q.3 Explain in detail DNA damage and repair 14
Q.4 What is transcription? Explain the transcription process in prokaryotes. 14
Q.5 Write in short on any Two of the following: 14
Explain the techniques and applications of DNA finger printing.
Evidence of Nucleic acid as genetic material.
Describe operon model with reference to arabinose operon.
Q.6 Write short notes on any TWO of the following: 14
Chromosome walking.
Give the brief account of nomenclature, classification, properties and types of
plasmids.
Post tranlational processing in eukaryotes.