M.Sc. (Semester II) (CBCS) Examination Nov/Dec-2018
Microbiology
MICROBIAL GENETICS
Time: 2½ Hours Max. Marks: 70
Instructions: Part Question 1 is compulsory.
Figures to the right indicate full marks.
Attempt any 4 questions from Part II.
Answer to the Part I and Part II are to be written in same answer booklet
only.
PART I
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
The function of t-RNA is
It "transfers" the information encoded in a gene to a ribosome
It forms part of a ribosome's structure.
It carries amino acids into a growing protein chain, as specified by codons
in the m-RNA
It permits messenger RNA to detach from the ribosome when protein
synthesis is complete
The exact point where nucleotides are being added to growing daughter
strands during DNA replication are called as
The origin The double helix
The template The replication fork
The conversion of a gene's nucleotide sequence into a protein is called as
The genome Gene expression
Transcription Translation
The initial source for all genetic variation is
Sexual reproduction Mutation
Conjugation Transformation
is the range of minimum set of genes required for life?
50-100 genes 250-350 genes
1000-1500 genes 1500-2000 genes
Biomolecules first arose by
Genetic evolution Randomly
Biological evolution Chemical evolution
Proofreading and mismatch repair in DNA is carried out by
DNA polymerase I DNA polymerase II
Exonuclease
SOS response brings to halt
DNA synthesis RNA synthesis
Protein synthesis Carbohydrates synthesis
In Archaeae translation is matching more to
Bacteria Yeasts
Eukaryotes Mitochondria
Page 2 of 2
SLR-VP-432
10) Production of antibiotics by microbes is mostly controlled by
Integrons Transposons
Plasmids Chromosomal genes
11) Most abundant class of organisms on earth are
Parasites of unicellular organisms
Plants
Insects
bacteria
12) In prokaryotes initiation codons always codes for
Methionine Cytocine
Glutamate N-Formyl methionine
13) Coordinated regulation of a function in bacteria is brought about by regulation
of synthesis of polycistronic mRNA regulated through
a single signal many signals coordinated by rho
double signal sigma factor
14) Sometimes you can see mRNA molecules growing in both directions of
dsDNA of bacterial chromosome; it is due to fact that
RNA polymerase can work in both direction
Opposite strands of a DNA sequence always code for two different genes
genes can be transcribed from opposite strands
DNA polymerase can work in both directions
PART II
Q.2 Describe in detail detection, purification amplification and rearrangement of
plasmids.
14
Q.3 Explain in detail the mechanism of DNA replication. 14
Q.4 Comment on "operon model with reference to lactose and arabinose operon." 14
Q.5 Write short answer on any two of the following:- 14
Structure of r RNA and t RNA
RFLP
Evidences of Nucleic acid as genetic material
Q.6 Write short notes on any 14
Terratogenesis
Genetic complementation
Organic evolution