M.Sc. Microbiology (Semester II) (CBCS) Examination, 2017
MICROBIAL GENETICS wateriI
Day Date: Wednesday, 19-04-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
N.B. Part- Questions NO.1 Compulsory.
Attempt any 4 questions from Part II.
Figures to the right indicate full marks.
Answer to the Part I and Part II are to be written in same
answer booklet only.

Q.1 Choose the correct alternative given in the bracket. 14
enzyme produces negative superhelicity and removes the
positive developed during replication.
Topoisomerase DNA gyrase
DNA polymerase I DNA polymerase II
Overlapping genes are found in
x 174 E. coli
T4 bacteriophage MS2
The law of purity of gametes is also known as Mendel's law of
heredity.
First Second Third Fourth
DNA sequences containing transposase gene flanked by inverted
repeats are called
IS elements Simple transposons
Composite transposons Phage elements
Synthesis of RNA primers for DNA chain elongation is carried out by

RNA polymerase RNA primase
DNA polymerase RNA transcriptase
In A form of DNA, one turn of helix consists of base pairs.
10 11 9.33 8
has the smallest chromosome?
Mycobacterium, E. coli, Salmonella, Mycoplasma
Page 1 of 2

SLR-RP 672
According to Oparin's theory source of energy for different chemical
reaction leading to formation of 'primordial soup'
From volcanoes, electrical discharges solar energy
None of these
Only heat from volcanoes
Only electrical discharges
Each DNA has reading frames.
6
10) Site specific recombination requires an enzyme recombinase which
identifies a unique DNA sequence of
2-20 bases, 200-2000 bases,
20-200 bases, None
11) In PCR DAN polymerase is used.
Type Type II, Type III, Type IV,
12) Most abundant class of organisms on earth are
Insects
Plants,
Parasites of unicellular organisms (UOPs)
Bacteria
13) The replicon encoding genes essential for the cell survival is called as

Genome Chromosome Codon Proteome
14) Cairn's model of DNA replication explains mode of DNA replication in
DNA.
Single stranded linear Double stranded linear
Single stranded Circular Double stranded circular
PART-II
Q.2 What is genetic complementation? Explain in detail intergenic and
intragenic complementation and add a note on cis-trans test of genetic
function.
14
Q.3 What is finger printing? Explain the techniques and applications of DNA
foot printing and DNA finger printing.
14
Q.4 Give the detailed account of detection, purification, amplification and
rearrangement of plasmids.
14
Q.5 Write in short on any Two of the following: 14
Types and properties of genetic code.
One gene one polypeptide hypothesis.
Discuss the rolling circle model of DNA replication.
Q.6 Write short notes on any TOW of the following: 14
Alternative forms of DNA.
Describe Operon model with reference to lac operon.
Post transcriptional in Prokaryotes.