Master of Science I (Zoology)Examination: Oct Nov 2016
Semester I (Old CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SN
721
Friday
18/11/2016
10:30 AM
to
01:00 PM
Tools And Techniques
In Biology
C
II
Instructions: Q.1, 2 6 are compulsory.
Answer any two questions from Q.3, 4 5
Total Marks: 70
Q.1 Multiple choice questions (per question 2 marks) 14
The resolution power of electron microscope is
0.3-0.5 nm 5-10 nm
1-3 nm 20-25 nm
The first human hormone produced by gene cloning was
thyroxine Insulin
Adrenalin Estrogen
Restriction enzymes are synthesized by
yeast cells only eukaryotic cells
bacteria only both b and c only
A piece of DNA that form hybrid and is used to identify a gene is called

vector bacteriophage
probe retrovirus
A method of separation of molecules on the bases of size, and their migration
through an electric field is called
chromatography sedimentation
flow cytometry electrophoresis
Units of radioactivity are
Curie Hertz
Ohms Celsius
The technique that uses antibodies to localize particular antigen in cytological
preparation is called
immunodiffusion immunity chromatography
immunoelectrophoresis immunocytochemistry
Q.2 Give and account on Ultracentrifugation and sub-cellular fractionation. 14
Page 1 of 2
Q.3 Answer the following:
Design and functioning of Tissue Culture Laboratory. 07
Application of Electrophoresis and its types. 07
Q.4 Explain the following:
Types of culture and its uses 05
Column chromatography 05
Cryopreservation of Cells 04
Q.5 Explain in short:
Immunocytochemistry and its applications 07
Capillary Culture Units 07
Q.6 Write short notes on any Four of the following: 14
Hybrid antibody
Radiolabel Techniques in Biology
Feeder Layers
Autoradiography
Mass Spectroscopy
Vectors