Master of Science I (Zoology)Examination: Oct Nov 2016
Semester I (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SN
716
Friday
18/11/2016
10:30 AM
to
01:00 PM
Tools And Techniques
In Biology
C
HCT 1.2
Instructions: Q.1, 2 6 are compulsory.
Answer any two questions from Q.3, 4 5
Total Marks: 70
Q.1 Multiple choice questions (per question 2 marks) 14
generate free radicals of acrylamide.
TEMED Acrylamide
Ammonium per Sulphate Agarose
Sample dissolved in glycine Chloride buffer at PH 8-9 in the upper
buffer exists as zwitterions.
Chloride Protein sample
Glycine both a b
More recently a term immunoblotting is introduced in
Southern blotting Western blotting
Northern blotting All of above
The radioactive element used to study newly synthesized protein is
Sodium Chlorine
Nitrogen Potassium
All the following are components of compound microscope except
stage clips fine adjustment
electron gun Binocular eye piece
Hybridomas are produced by fusion of
Selected lymphocytes lymphocytes tumour cell
Tumour cells Hela cells Hela cells plants cells
The locating agent of amino acid is
Diazo reagent Amphoteric oxides
Neutral acids Ninhydrin spray
Q.2 What is Chromatography? Describe the principle of column chromatography and
its applications.
14
Page 1 of 2
Q.3 Answer the following:
Design and functioning of tissue culture laboratory. 07
Freeze Drying and freeze fracturing technique. 07
Q.4 Explain the following:
DNA cloning 05
Cell Hybrids and its Application 05
X-rays in Biology 04
Q.5 Explain in short:
Cryotomy 07
Radiolabel techniques in biology 07
Q.6 Write short notes on any Four of the following: 14
Transmission Electron Microscope
Cryopreservation of cells
Autoradiography
MALDI
Ultracentrifugation
NMR