M.Sc. (Semester (CBCS) Examination Oct/Nov-2017
Zoology
TOOLS AND TECHNIQUES IN BIOLOGY
Day Date: Saturday, 18-11-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Question number 2 and 6 are compulsory.
Attempt any two from Question No. 4 and 5.
Q.1 Multiple choice questions (Per question 2 marks): 14
The radioactive element used to study newly synthesized proteins

Sodium Chlorine
Nitrogen Potassium
Which would be best to separate a protein that binds strongly to its
substrate?
Gel filtration Affinity chromatography
Cation exchange Anion exchange
Immunoblotting is introduced in technique.
Southern blotting Western blotting
Northern blotting All of above
ELISA is used
Separate viral RNA Purify proteins
Isolate DNA Identify specific proteins
The best technique to separate isoenzymes is
Paper chromatography Electrophoresis
Microscopy Thin layer chromatography
Shadow casting is used in
TEM SEM
Phase contrast Fluorescence
Hybridomas are produced by fusion
Selected lymphocytes Iymphocytes tumour cell
Tumour cells Hela cells Hela cells plants cells
Q.2 Discuss the steps in Proteomics. 14
Q.3 Answer the following.
Describe the technique used to produce Monoclonal antibodies. 07
Comment on the uses of cell hybrids? 07
Q.4 Explain the following.
Lasers in Biology. 05
Autoradiography 05
Flowcytometry 04
Q.5 Explain in short
Agarose Electrophoresis. 07
Column Chromatography. 07
Q.6 Write short notes on any four of the following: 14
Radioimmunoassay.
FTIRspectrometer
Cell transformation.
Cryotomy.
Ultracentrifugation.
rays in Biology.