M.Sc. (Semester (CBCS) Examination Nov/Dec-2018
Zoology
TOOLS AND TECHNIQUES IN BIOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw diagrams wherever necessary.
Q.1 Choose the correct alternatives for given multiple choice question:- 14
The rate of migration of DNA within an agarose gel electrophoresis
technique is primarily based on the
Number of DNA fragments Size of DNA fragment
Size of wells in gel Negative charge of DNA
The RCF in centrifugation stands for force.
Regular Centripetal Relative Centripetal
Regular Centrifugal Random Centrifugal
The tracking dye used in electrophoresis is
Bromophenol blue Blue dextran
Phenol red Neutral red
due to excitation by radioactivity is known as scintillation.
Fluorescence Transmission
Scattering Emission
The absorption maximum for proteins is at nm, which is used
in UV-V is spectrophotometry.
260 280
320 340
is used as spraying reagent in paper chromatography for
visualization of amino acids.
Iodine Basic fuchsine
Ninhydrin Aniline
is not used as gradient maker in density gradient
centrifugation.
Sucrose Glycerol
CsCI Maltose
It was in 1907 Ross Harrison first developed a techniques.
Frog tissue culture Human tissue culture
Mouse tissue culture Chick tissue culture
Sterilization by dry heat carried out at a minimum temperature of
oC for one hour.
200 160
300 400
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SLR-VS-502
10) is used as a pH indicator in animal tissue culture artificial media.
Crystal violet Bromophenol blue
Phenol red Both a and b
11) is the phenomenon of the change in phenotype due to the
acquirement of new genetic material.
Conjugation Transformation
Replication Translation
12) For cell dissociation of monolayer culture enzyme is mostly
used.
Trypsin Amylase
Cellulase All of the above
13) Normal animal cell shows growth in artificial medium.
Multiple layer Indefinite
Monolayer None of these
14) The temperature for cryopreservation is oC.
-200 -158
-196 -160
Q.2 Answer the following:- (Any Four) 08
Define spectrophotometer.
What is mean by Proteomics?
Define Fusogen with example.
Define Freeze drying.
What is Radioactivity?
Write notes (Any two) 06
Add a note on Laser in Biology.
Explain Monolayer.
Write a note on Fusion in different cell-cycle phase.
Q.3 Answer the following:- (Any two) 08
Explain in detail Transmission electron microscope.
What is Cell characterization and explain it.
Write a note on Cell hybrid with application.
Answer the following:- (Any one) 06
Explain in detail about Hybridoma technology.
Define chromatography and add a note on thin layer chromatography.
Q.4 Answer the following:- (Any two) 10
Explain in detail FTIR.
What is mean by Cryopreservation? Explain its techniques.
Add a note on cell culture media.
Answer the following:- (Any one) 04
Explain in detail regarding cell viability testing.
Add a note on freeze drying and freeze fracturing.
Q.5 Answer the following:- (Any two) 14
Define electrophoresis and add a note on different types of electrophoresis.
Add a note on two different ways for measurement of radioactivity.
Define centrifuge and explain in detail types of centrifuge.