FACULTY OF PHARMACY
B. Pharmacy 4/4 II-Semester (Main) Examination, April 2017
Subject Pharmaceutical Biotechnology
Time 3 Hours Max. Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Give a brief account of
Cloning strategies
DNA ligases
(iii)Restriction endonucleases
OR
Explain the manufacture of Insulin by rDNA technology.
2 Explain the principle involved in microbiological assay of Antibiotics. Add a note on
one level and two level factorial assay.OR
Define Immobilization. Explain the methods for immobilization of Enzymes. Add a
note on its applications.
3 Give an account of preparation and standardization of Bacterial vaccines.
OR
Give a brief account of
Viral vaccines
Immunodiagnostics
4 Give an account of collection, processing and storage of whole human blood. Add a
note on the preparation of dried human plasma.
OR
What are the ideal requirements of plasma substitute? Write the preparation of
DEXTRAN.
5 Give an account of microbial Transformation of steroids.
OR
Give a brief account of
Animal cell culture Techniques
Hybridoma Technology