FACULTY OF PHARMACY
B. Pharmacy 4/4 II-Semester (Main) Examination, March April 2016
Subject Pharmaceutical Biotechnology
Time 3 Hours Max. Marks: 70
Note: Answer All questions. All questions carry equal marks.
Give an account of production of Insulin by rDNA technology.
OR
Write brief account on
Restriction Endonucleases
Cloning strategies
Give a detailed account of various methods for immobilization of Enzymes. Add a
note on their applications.
OR
Give a brief account on
Aeration in fermentors
Scale up of fermentors
Batch and continuous fermentations
Define and classify vaccines. Write the preparation and standardization of any
one bacterial vaccine.
OR
Give a brief account on
Preparation and purification of Diptheria antitoxin
Immunodiagnostics
Give an account of collection, processing and storage conditions of whole
Human Blood and concentrated Human RBC. Add a note on the preparation and
sterilization of Human Fibrin foam.
OR
What are the ideal requirements of plasma substitute? Write the fermentative
production of Dextran.
Give an account of production of monoclonal antibodies. Add a note on their
applications.
OR
What is the significance of microbial transformation of steroids? Explain the type
and methods of transformation mediated by micro organisms.