M.Sc. (Semester II) (CBCS) Examination Mar/Apr-2018
Biotechnology
MOLECULAR CELL PROCESSING
Time: 2½ Hours
Max. Marks: 70
Instructions: Section I is compulsory. Answer any Four questions from section II. All question carry equal marks. Figures to the right indicate full marks. Draw neat and labeled diagram.
Section I
Q.1

Rewrite the sentence after choosing the correct from the given
07
In prokaryotes, the first amino acid in the polypeptide chain is
Methionine
N-formyl methionine
Both a b
None
In prokaryotes, the ribosomal binding site on mRNA is
Hogness sequence
Shine-Dalgarno
Eukarya
All of these
Transcription termination occurs by
Rho-dependent
Rho-independent
Sigma factor
Both a b
RNA required for protein synthesis.
tRNA
mRNA
rRNA
all of these
RNA primer necessary for DNA replication
The RNA primer is necessary for the activity of DNA ligase.
The RNA primer creates the and ends of the strand.
DNA polymerase can only add nucleotides to RNA molecules.
DNA polymerase can only add nucleotides to an existing strand.
During the process of transcription, of the following is produced.
H2O
ATP
mRNA
DNA
During DNA replication the formation of bond takes place between
Bases
Sugar and bases
Phosphates and bases
Sugars and phosphates

Define the terms.
07
Group II introns
Glycosylation
Holiday Model
30S Ribosome
16S rRNA
SOS repair
rec BCD pathway
Page 2 of 2
SLR-UE-91
Section II
Q.2
Answer any four of the following:- Explain the process of eukaryotic RNA editing with neat labeled diagram.
14
Q.3
What is mutation? Explain mismatch and SOS DNA repair mechanism with neat labeled diagram.
14
Q.4
Describe the structure, assembly and function of each subunit of DNA Pol III with neat Labeled diagram.
14
Q.5
Answer any two from the following:-
Describe the process of transcription in prokaryotes.
Explain the structure of eukaryotic gene.
Explain difference between eukaryotic and prokaryotic DNA replication.
14
Q.6
Write short notes on. (Any two)
14
E. Coli DNA Pol I
Protein folding
tRNA, mRNA and rRNA